Evaluation of growth and motility in non-photosynthetic Azospirillum brasilense exposed to red, blue, and white light
- PDF / 881,751 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 51 Downloads / 164 Views
ORIGINAL PAPER
Evaluation of growth and motility in non‑photosynthetic Azospirillum brasilense exposed to red, blue, and white light Molina Romina1 · López Gastón1 · Rodríguez Belén1 · Rosas Susana1 · Mora Verónica1 · Cassán Fabricio1 Received: 22 August 2019 / Revised: 28 December 2019 / Accepted: 4 February 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Azospirillum brasilense is a non-photosynthetic rhizobacterium that promotes the growth of plants. In this work, we evaluated the effects of different light qualities on the growth, viability, and motility in combination to other culture conditions such as temperature or composition of the culture medium. Exponential cultures of A. brasilense Az39 were inoculated by drop-plate method on nutritionally rich (LB) or chemically defined (MMAB) media in the presence or absence of Congo Red indicator (CR) and exposed continuously to white light (WL), blue light (BL), and red light (RL), or maintained in dark conditions (control). The exposure to BL or WL inhibited growth, mostly in LB medium at 36 °C. By contrast, the exposure to RL showed a similar behavior to the control. Swimming motility was inhibited by exposure to WL and BL, while exposure to RL caused only a slight reduction. The effects of WL and BL on plant growth-promoting rhizobacteria should be considered in the future as deleterious factors that could be manipulated to improve the functionality of foliar inoculants, as well as the bacterial effects on the leaf after inoculation. Keywords Azospirillum · Non-photosynthetic rhizobacteria · Red light · Blue light · White light · Bacterial growth · Swimming motility
Introduction Light is an essential environmental factor for the development and continuity of life. Certain organisms, known as “photosynthetic” organisms, have developed the ability to capture light and transform it into chemical energy, a transformation through which they regulate metabolic functions Communicated by Erko Stackebrandt. Molina Romina and López Gastón have contributed equally to this work. Mora Verónica and Cassán Fabricio have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01829-8) contains supplementary material, which is available to authorized users. * Cassán Fabricio [email protected] 1
Laboratorio de Fisiología Vegetal y de la Interacción Planta‑microorganismo, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, Córdoba, Argentina
(Elías-Arnanz et al. 2011). However, light can also cause photophysical and photochemical damage to the structure of nucleic acids, proteins, and lipids, and/or trigger the synthesis of highly toxic metabolites (van der Horst and Hellingwerf 2004). Particularly, the wavelengths responsible for such lethal effects (Christie et al. 1999; Hitomi et al. 2000) are found in the violet (380–450 nm) and blue (440–480 nm) parts of the visible spectrum. In presence of visible light, oxygen, and certain photosensi
Data Loading...
