Evolving Trends in Transition Metal-Modified Receptor Design and Function
Nature elegantly invokes transition metal ions in a number of fundamental biomolecular processes from guiding protein folding and scaffolding tertiary structures to essential roles in information transmission through ligand binding, electron transfer, and
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Abstract Nature elegantly invokes transition metal ions in a number of fundamental biomolecular processes from guiding protein folding and scaffolding tertiary structures to essential roles in information transmission through ligand binding, electron transfer, and catalysis. Often inspired by such natural systems, innovative transition metal-modified receptors are beginning to emerge with similar functions that hold substantial promise as components of next generation biosensors. This chapter aims to highlight some recent advances in the development of transition metalmodified synthetic receptors. Specifically, systems that incorporate organometallic, monometallic, and supramolecular coordination complexes are reviewed. The text will cover transition metal scaffolded peptide receptors, allosteric supramolecular enzyme mimics, and integrated monolayer-based electroactive receptor systems. Keywords Allosteric Metalloreceptor
supramolecular
systems,
Ferrocene
bioconjugates,
Contents 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Transition Metal Complexes as Scaffolds in Receptor Design . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1 Metal-Directed Peptide Assemblies for Molecular Recognition . . . . . . . . . . . . . . . . . . . . 2.2 Metalloreceptor Scaffolds in Differential Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Functional Allosteric Supramolecular Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Monolayer Receptors with Integrated Metal-Based Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.1 Ferrocene–DNA Conjugates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2 Peptide-Functionalized Metalloreceptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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P.A. Bertin (*) Ohmx Corporation, 1801 Maple Avenue, Suite 6143, Evanston, IL 60201, USA e-mail: [email protected] S.A. Piletsky and M.J. Whitcombe (eds.), Designing Receptors for the Next Generation of Biosensors, Springer Series on Chemical Sensors and Biosensors (2013) 12: 239–260 DOI 10.1007/5346_2012_20, # Springer-Verlag Berlin Heidelberg 2012, Published online: 27 June 2012
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Abbreviations Ala Arg Asp Bpy BSA Cys DNA ELISA ESI-MS FRET Glu His Leu NMR PCR Pro RNA SAM Ser Trp Tyr
Alanine Arginine Aspartic acid Bipyridine Bovine serum albumin Cysteine Deoxyribonucleic acid Enzyme lin
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