Experimental Study of the Effect of Biological Matrixes with Stabilized and Non-Stabilized Platelets on Reparative Proce
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Cell Technologies in Biology and Medicine, No. 3, November, 2020
Experimental Study of the Effect of Biological Matrixes with Stabilized and Non-Stabilized Platelets on Reparative Process in the Wound Equivalent to Deep Burn N. V. Borovkova, M. S. Makarov, I. N. Ponomarev, Yu. V. Andreev, M. V. Storozheva, and A. A. Budaev
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 170-177, September, 2020 Original article submitted April 16, 2020 We studied reparative effect of platelet-filled biological matrixes in the treatment of mice with wounds equivalent to deep burn. The wound coatings were based on decellularized dermal matrix without platelets (control), with native platelets, and with platelets stabilized with 2.5 µM nanosilver. In 3 days, the epithelial layer and derma were absent in all groups and extensive scab was formed. Dermal matrix with platelets simulated intensive migration of macrophages and fibroblasts to the wound bottom; in the control group, this migration was absent. In 14 days, granulation tissue appeared in the wound bottom in animals of all groups; in the experimental groups, the number of vessels was 2-4-fold higher than in the control, though the number of inflammatory cells in experimental groups remained high. On day 21, the scab on the most of the wound area was absent in all animals of the experimental groups and epithelialization and hair growth were pronounced, comparing to control. Nevertheless, in experiment dermal layer was not already completed, inflammation reaction remained. Key Words: dermal matrix; platelets; inflammation; cell migration; epithelialization Autodermoplasty remains the basic approach of the therapy of deep burn wounds [8,10,13]. Shortening of the time of preparing to surgery and improvement of clinical outcomes are still pressing problems. The aim is to stimulate regenerative processes in the wound, to accelerate tissue regeneration, and to prevent the growth of pathogenic microbes. In previous studies, we showed that dermal matrix (DM), a biological coating prepared from devitalized allogenic split skin flap, produced favorable effect on the course of the wound process [7]. The maximum growth-simulating reparative effect was provided by DM combined with allogenic progenitor cells due to their paracrine effect. However, after enactment of the Federal Law of the Russian Federation No. 180 (On Biomedical Cell Products; June 23, 2016), introduction of this techN. V. Sklifosovsky Research Institute for Emergency Medicine, Moscow Healthcare Department, Moscow, Russia. Address for correspondence: [email protected]. M. S. Makarov
nique into clinical practice is problematic because it implies the use of cultured progenitor cells. This necessitates the search for alternative sources of growthstimulating factors and human platelets seem to be a promising solution. Platelets are unique highly differentiated cells playing an important role in various biological processes [1,3]. Diverse functions of platelets are determined by the presence of a gre
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