Exploration of haloarchaea for their potential applications in food industry
- PDF / 2,117,935 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 100 Downloads / 272 Views
ORIGINAL PAPER
Exploration of haloarchaea for their potential applications in food industry C. Desai1 · P. Patel1 · A. R. Markande1,2 · K. Kamala3 · P. Sivaperumal4 Received: 26 November 2019 / Revised: 8 May 2020 / Accepted: 11 May 2020 © Islamic Azad University (IAU) 2020
Abstract Extremophiles are a major source for many industrially important biomolecules. Haloarchaea have been extensively studied for their ability to grow in extreme environments and the array of metabolites especially osmolytes they produce with resistance to extreme physical and chemical conditions. In the present study, we have studied the haloarchaeal isolates for their ability to produce industrially important biomolecules like—proteases, amylases, surfactants, antifreeze and antidesiccation proteins. The haloarchaea used in this study were isolated from fish sauce fermentation broth and belonged to Halorubrum sp. SS1 (KY053875.1), Halobacterium sp. SS2 (KY053876.1), Halococcus sp. AMS1 (KU995303.1), Halorhabdus sp. AMS6 (KU995310.1) and Halobacterium sp. SFF3 (KY053871.1). All the archaea isolated in this report had negligible amylase and surfactant activities but showed marginal protease activity. The isolates were also found to be producing significant antifreeze protein stable till 192 h and desiccation tolerant till 240 min of studies. This is the first report of microbial extracellular proteins exhibiting antidesiccation and antifreeze properties, thus introducing a new family of industrially important microbial proteins. These applications have immense potential in food preservation and processing. Keywords Haloarchaea · Antidesiccation · Antifreeze · Survival · Biofilm · Adhesion
Introduction Halophiles, the organisms that thrive in high salt concentration, are a type of extremophilic organisms. Halophiles can be found anywhere with a concentration of salt five times or greater than the salt concentration of the ocean (~ 3.4%). The high salt concentration in these saline environments limits Communicated by Jing Chen. * A. R. Markande [email protected] 1
C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Bardoli Mahuva Road, Tarsadi, Surat, Gujarat 394 350, India
2
Department of Biological Sciences, P.D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, Anand, Gujarat 388 421, India
3
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603 203, India
4
Center for Environmental Nuclear Research, Directorate of Research, SRM Institute of Science and Technology, Kattankulathur 603 203, India
the availability of oxygen for respiration, making the conditions stressful for most of the microorganisms except haloarchaea. Haloarchaea (particularly family Halobacteriaceae comprising 15 genera) only recently have been determined for its abundance in natural populations within last two decades (Santos and Da Costa 2002). Most archaea are highly adapted for extreme chemical and/or physi
Data Loading...
