Expression of angiopoietin-like protein 2 in ovarian tissue of rat polycystic ovarian syndrome model and its correlation
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(2020) 18:94
RESEARCH
Open Access
Expression of angiopoietin-like protein 2 in ovarian tissue of rat polycystic ovarian syndrome model and its correlation study Dandan Wang1, Yihong Guo2* , Shujuan Chai1, Ke Shen1, Yanchun Li1 and Ruiqin Zhao1
Abstract Background: This study investigated the expression of angiopoietin-like protein 2 (ANGPTL2) in the tissues of rat models of polycystic ovary syndrome (PCOS) and its correlation with PCOS. Methods: Six-weeks-old female specific pathogen-free rats (n = 60) were divided into blank control, PCOS model, and metformin groups (n = 20/group). After 21 days of metformin intervention, the serum sex hormones, fasting blood glucose, fasting insulin, and insulin resistance (IR) of rats in each group were measured. The mRNA levels of ANGPTL2, Foxol, and Akt in the ovarian tissues were monitored by real-time fluorescence quantitative PCR. Results: Compared with the control group, the levels of serum sex hormones, fasting blood glucose, fasting insulin, and IR in the model group showed significant increases, and the levels of ANGPTL2, Foxol, and Akt in the ovarian tissue also showed significant increases. Compared with the PCOS group, the serum sex hormones, fasting blood glucose, fasting insulin, and IR of rats in the metformin group were significantly decreased, and the levels of ANGP TL2, Foxol, and Akt in ovarian tissues also showed significant decreases. Conclusions: These findings suggest that ANGPTL2 might participate in the development of PCOS through the PI3K/Akt signaling pathway. Metformin improves IR by reducing the expression of ANGPTL2, thus improving the endocrine environment of PCOS and might change the disease outcome. Keywords: Polycystic ovary syndrome, PI3K/Akt, Angiopoietin-like protein 2, Metformin
Background Polycystic ovary syndrome (PCOS) is one of the most common endocrine, metabolic disorders in women of childbearing age, and it is regarded as a frequent cause of ovulation disorders and infertility in women [1], as well as cardiovascular disease, diabetes, and endometrial cancer [2]. The incidence of PCOS has been reported to be 4–10% [3]. At present, there is no unified opinion about the etiology of PCOS. Insulin resistance (IR) plays an important role in the development of PCOS [4]. Glintborg et al. [5] showed that * Correspondence: [email protected] 2 Reproductive and Genetic Hospital, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China Full list of author information is available at the end of the article
women with PCOS have ovarian dysfunction, indicating systemic and local IR in the ovarian tissue of PCOS patients. PCOS ovarian IR mainly occurs due to the insulin signal transduction pathway, which in turn is closely related to the impairment of key molecules of insulin, Lphosphatidylinositol 3-kinase (PI3K), and glucose transporter 4 signaling pathway [6, 7]. PI3K/serine-threonine protein kinase (Akt) is an insulin-related signaling pathway, and insulin activates the downstream PI3K for regulating glucose metabolis
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