Extruded polysaccharide/protein matrix from Arthrospira platensis cultures mediated silver nanoparticles biosynthesis an
- PDF / 4,401,009 Bytes
- 17 Pages / 595.276 x 790.866 pts Page_size
- 34 Downloads / 170 Views
ORIGINAL ARTICLE
Extruded polysaccharide/protein matrix from Arthrospira platensis cultures mediated silver nanoparticles biosynthesis and capping Ragaa A. Hamouda1,2 · Mervat H. Hussein3 · Abdel Monsef A. Elhadary4 · Muhammad A. Abuelmagd3 Received: 2 May 2020 / Accepted: 17 June 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Herein, Silver nanoparticles (AgNPs) have been synthesized and capped using secreted polysaccharide-protein matrix (EPS) from Arthrospira platensis cultures (A-EPS) and compared with EPS extracted from 1.0 K Gy gamma-irradiated A. platensis cultures (IA-EPS). Gamma irradiation enhanced EPS secretion and modified its biochemical composition. A. platensis cultures have been exposed to two acute gamma irradiation doses of 1.0–2.0 K Gy, EPS secretion was scaled up in response to gamma irradiation from 0.559 g L−1 at non-irradiated culture to 0.752, 0.690 g L−1 at 1.0–2.0 K Gy, respectively. Saccharide-protein nature of EPSs was confirmed via chemical, UV Visible spectrophotometer, and Fourier-transform infrared spectroscopy (FT-IR), revealed an increment of protein content in (IA-EPS). Hetero-polymeric constituents were elucidated using HPLC analysis which revealed seven monomers in the saccharide portion of (IA-EPS). AgNP biosynthesis was fabricated in alkaline medium whereas IA-EPS-mediated AgNPs (IEPS-AgNPs) exhibited a stronger UV absorbance than A-EPS-mediated AgNPs (EPS-AgNPs) at pH 9.0 and 2.0 mg L−1. Synthesized AgNPs displayed the difference in coating nature and its aggregation under Transmission Electron Microscope (TEM) whereas (IEPS-AgNPs) displayed mono-dispersed nanoparticles capped by protein layers meanwhile (EPS-AgNPs) exhibited poly-dispersed ones capped with a faint sheath of EPS. FT-IR analysis illustrated the capping mechanism for both EPS-AgNPs and IEPS-AgNPs. Keywords Silver nanoparticles · Arthrospira platensis · Extracellular polymeric matrix · Reducing-capping agent · Gamma irradiation
Introduction Nanotechnology is a fast-growing unexpectedly technology in which engineered nano-scaled materials (silver nanoparticles) are synthesized for an unlimited range of applications in different fields of current science such as agriculture, biomedical and pharmaceutical field (Mehta et al. 2014; Huq 2020). Nanoparticles attract researchers attention due to its * Ragaa A. Hamouda [email protected] 1
Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Jeddah, Kingdom of Saudi Arabia
2
Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt
3
Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt
4
Biological Application Dept, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt
characteristic biological, chemical and physical functional properties when compared to other large-sized particles as a result of its small size which ranges from 1 to 100 nm, large surface area and high reactivity (Kanmani and Lim 2013; Singh et al. 20
Data Loading...
