Finding new addictive QTL for yield traits based on a high-density genetic map in hybrid rice
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ORIGINAL PAPER
Finding new addictive QTL for yield traits based on a high‑density genetic map in hybrid rice Miao Zhang1 · Zheng‑ping Zhou1 · Yu‑yu Chen1 · Yong‑run Cao1 · Chen‑wei Deng1 · Pao Xue1 · Xiao‑deng Zhan1 · Shi‑hua Cheng1 · Li‑yong Cao1 · Ying‑xin Zhang1 Received: 14 May 2020 / Accepted: 24 September 2020 © Springer Nature B.V. 2020
Abstract Rice is one of the most important food crops in the world. To discover the genetic basis of yield components in super hybrid rice Nei2You No.6, 386 recombinant inbred sister lines (RISLs) were obtained for mapping quantitative trait loci (QTL) responsible for grain yield per plant, number of panicles per plant, grain number per panicle and 1000-grain weight. Using whole genome re-sequencing, a high-density linkage map consisting of 3203 Bin markers was constructed with total genetic coverage of 1951.1 cM and average density of 0.61 cM. A total of 43 yield-related QTL were mapped to all 12 chromosomes, and each explained 2.40–10.17% phenotypic variance, indicating that the medium and minor effect QTL are genetic basis for high yield of Nei2You No.6. With positive effect, 28 out of the 43 QTL are inherited from the maintainer line (Nei2B). Nine loci, qGYP-6b, qGNP-6c, qNP-7, qTGW-1a, qTGW-5, qTGW-7, qTGW-10b, qTGW-10c and qTGW-12 showed stable effects across multiple environments. Five of these nine QTL were co-located with previously reported QTL, and four novel locus, qNP-7, qGNP-6c, qTGW-7 and qTGW-12, were firstly identified in the present study. Subsequently, qNP-7, qTGW-7 and qTGW-12 were validated using corresponding paired sister lines which differed only in the target genome region. The recombinant inbred sister lines is an effective tool for mapping and confirming QTL of yield-associated traits. Newly detected QTL provide new resource for investigating genetics of yield components and will accelerate molecular breeding in rice. Keywords Recombinant inbred sister lines · High-density linkage map · Yield · Quantitative trait loci · Oryza sativa L. Abbreviations FY Fuyang LS Lingshui RISL Recombinant inbred sister line GNP Grain number per panicle GYP Grain yield per plant NP The number of panicles Miao Zhang and Zheng-ping Zhou have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00669-2) contains supplementary material, which is available to authorized users. * Li‑yong Cao [email protected] * Ying‑xin Zhang [email protected] 1
State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311401, China
QTL Quantitative trait loci SNP Single nucleotide polymorphism TGW Thousand grain weight
Introduction Rice (Oryza sativa L.) is the staple food for more than half of the global population. With the dwindling available arable land and the increasing population, food security has become a worldwide concern. Increasing grain yield per plant (GYP) is an effective way to solve thi
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