First report of the complete genomic sequences of strawberry mild yellow edge virus and strawberry vein banding virus is
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First report of the complete genomic sequences of strawberry mild yellow edge virus and strawberry vein banding virus isolated in Japan Yoshiaki Takamura1 · Satsuki Yamaryo1 · Wei‑Qin Wang1 · Yutaro Neriya1 · Emiko Suzuki2 · Kiyoshi Namai2,4 · Asad Ali3 · Hisashi Nishigawa1 · Tomohide Natsuaki1 Received: 2 March 2020 / Accepted: 6 April 2020 © The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature 2020
Abstract Strawberry vein banding virus (SVBV) and strawberry mild yellow edge virus (SMYEV) have caused disease in strawberries for many years in Japan, but little is known about their molecular properties. We thus determined the complete genomic sequence of an isolate of each virus from Tochigi Prefecture. A nucleotide sequence analysis showed that SVBV is closely related to Chinese isolates, whereas SMYEV is closely related to German and Argentine isolates. This is the first report describing the complete genome sequences of SVBV and SMYEV isolated in Japan. Moreover, we constructed an infectious SMYEV clone, which induced mild yellowing on inoculated strawberry plants. Keywords Strawberry · Caulimovirus · SVBV · Potexvirus · SMYEV Strawberry is an important fruit crop, with 12.9 million tons of the fruit harvested worldwide in 2017 (Food and Agriculture Organization of the United Nations; https://www. fao.org/faostat/en/). Globally, approximately 30 viruses have been isolated from strawberry. When coinfected with a mixture of viruses, strawberry plants can often develop severe symptoms due to synergistic interactions between the viruses, whereas infection with a single virus often leads to The DDBJ/EMBL/GenBank accession numbers for the SVBV-T, SMYEV-T1, and SMYEV-T2 sequences are LC315804, LC515235, and LC515236, respectively Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10327-020-00947-x) contains supplementary material, which is available to authorized users. * Yutaro Neriya [email protected]‑u.ac.jp 1
School of Agriculture, Utsunomiya University, Mine‑machi 350, Utsunomiya, Tochigi 321‑8505, Japan
2
Tochigi Prefectural Agricultural Experiment Station, Kawaraya‑cho 1080, Utsunomiya, Tochigi 320‑0002, Japan
3
Department of Plant Pathology, The University of Agriculture, Peshawar 25130, Khyber Pakhtunkhwa, Pakistan
4
Present Address: Tochigi Agricultural College, Kamikomoriya‑machi 1145‑1, Utsunomiya, Tochigi 321‑3233, Japan
mild symptoms or a latent infection (Martin and Tzanetakis 2013). Strawberry vein banding disease has been known for several decades, and in the 1980s, the causal agent was identified as strawberry vein banding virus (SVBV) (Frazier and Morris 1987), which belongs to the genus Caulimovirus of the family Caulimoviridae. The viral genomic DNA encodes six open reading frames (ORFs) in about 7.8-kbp circular double-stranded DNA, and the virus particle is 40–50 nm in diameter (Petrzik et al. 1998a). Additionally, SVBV has been detected in Australia, North and South America
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