Identification and full genomic sequence of nerine yellow stripe virus
- PDF / 789,120 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 254 Views
ANNOTATED SEQUENCE RECORD
Identification and full genomic sequence of nerine yellow stripe virus Naomi Beddoe1 · Ian P. Adams2 · Sam McGreig2 · Alec Forsyth1 Received: 8 May 2020 / Accepted: 14 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract This study reports the first complete genome sequence of nerine yellow stripe virus (NeYSV, GenBank MT396083). The genome consists of 10,165 nucleotides, excluding the 3’-terminal poly(A) tail. A single open reading frame encodes a large polyprotein of 3294 amino acids with typical potyvirus features. The nuclear inclusion b and coat protein region shares 95% identity with a previously reported partial NeYSV sequence (NC_043153.1). Phylogenetic analysis of the polyprotein amino acid sequence showed that NeYSV clustered with hippeastrum mosaic virus (HiMV YP_006382256.1). Nerines are native to southern Africa, are valued for their striking flowers, and are popular in the horticultural and floristry industries. They are grown commercially in Europe, are traded internationally, and are becoming increasingly important in the UK. Nerines are bulbous flowering plants of the family Amaryllidaceae and include hardy and tender varieties. Due to the vegetative nature of propagation, accumulated virus infection can persist through generations. Nerine yellow stripe virus (NeYSV), a virus in the family Potyviridae, was previously identified (Wendy Monger personal communication to Andrew Eames, 2007) in nerine plants growing in the UK, but the full genome sequence was not determined. The host range of NeYSV appears to be confined to the Amaryllidaceae, with infections reported in Handling Editor: Ralf Georg Dietzgen. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04776-3) contains supplementary material, which is available to authorized users. * Alec Forsyth [email protected] Naomi Beddoe [email protected] Ian P. Adams [email protected] Sam McGreig [email protected] 1
Canterbury Christ Church University, North Holmes Road, Canterbury, Kent CT1 1QU, UK
Fera Science Limited, The National Agri-Food Innovation Campus, Sand Hutton, York YO41 1LZ, UK
2
multiple countries and symptoms including mild to severe mosaic, darker green oval spots, chlorotic/yellow stripes on leaves/stalks, and occasional flower breaking [1–5]. NCBI currently holds partial sequences of NeYSV derived from various plant genera. These include two sequences from a nerine plant (Netherlands, NC_043153.1, EF362621.1), seven partial sequences from Crinum genus plants (USA, MG012805.1 and India, KJ886934.2, KJ886933.2, KM066971.1, KM066970.1, KM066969.1, KM066968.1), two partial sequences from Amaryllis genus plants (USA, JX865782.1 and New Zealand, FJ618537.1), and one partial sequence each from a Stenomasson genus plant (Netherlands, EU042758.1), a Hymenocallis genus plant (Netherlands, EF362622.1), and a Cyrtanthus genus plant (previously Vallotta genus) (New Zealand, DQ407932.1). A pla
Data Loading...
