Gene Expression Analysis Methods and Protocols
This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplex
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Nalini Raghavachari Natàlia Garcia-Reyero Editors
Gene Expression Analysis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Gene Expression Analysis Methods and Protocols
Edited by
Nalini Raghavachari Division of Geriatrics and Clinical Gerontology, National Institute on Aging, Bethesda, MD, USA
Natàlia Garcia-Reyero Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, MS, USA
Editors Nalini Raghavachari Division of Geriatrics and Clinical Gerontology National Institute on Aging Bethesda, MD, USA
Nata`lia Garcia-Reyero Environmental Laboratory US Army Engineer Research and Development Center Vicksburg, MS, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7833-5 ISBN 978-1-4939-7834-2 (eBook) https://doi.org/10.1007/978-1-4939-7834-2 Library of Congress Control Number: 2018941461 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Rapid advances in genomic technologies and computational tools are revolutionizing the field of transcriptomics, which is the study of the transcriptome or the genes transcribed from genomic DNA. The applications of these techniques are endless. From understanding development and disease to environmental monitoring, or community analysis, the published manuscripts using gene expression to tackle different questions have not stopped and increasing expone
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