Gene polymorphism of chalcone isomerase influence the accumulation of flavonoids in licorice ( Glycyrrhiza spp.)
- PDF / 1,980,488 Bytes
- 15 Pages / 547.087 x 737.008 pts Page_size
- 27 Downloads / 193 Views
(0123456789().,-volV) (0123456789().,-volV)
RESEARCH ARTICLE
Gene polymorphism of chalcone isomerase influence the accumulation of flavonoids in licorice (Glycyrrhiza spp.) Xiaodong Zhang . Shaokai Tian . Lin Qi . Wendong Li . Jiaming Hou . Lin Yang . Zhixin Zhang . Ying Liu
Received: 30 December 2019 / Accepted: 23 September 2020 Springer Nature B.V. 2020
Abstract Licorice has been used in traditional Chinese medicine for more than 2000 years. Chalcone isomerase (CHI) is the second rate-limiting enzyme involved in the biosynthetic pathway of flavonoids in licorice. The purpose of this study is to analyze the CHI gene polymorphism and identify CHI haplotypes associated with high/low flavonoid contents. Five Glycyrrhiza uralensis samples with the high flavonoids contents and five Glycyrrhiza inflate samples with low flavonoids contents were selected to clone the CHI cDNA sequences. The CHI haplotypes related to the accumulation of flavonoids were determined and induced to express in Pichia pastoris GS115. Enzymatic reactions and HPLC method were used to verify the functions of the specific CHI haplotypes. 100 CHI cDNA sequences with a full length of 690 bp were obtained and 48 haplotypes were determined. Haplotype 1 and 31 are the major haplotypes in groups Xiaodong Zhang, Shaokai Tian, and Lin Qi these authors have been contributed equally to this paper. X. Zhang S. Tian J. Hou L. Yang Z. Zhang Y. Liu (&) School of Life Sciences, Beijing University of Chinese Medicine, Yangguang South Street, Fangshan District, Beijing 102488, China e-mail: [email protected] L. Qi W. Li Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing Institute for Drug Control, Beijing, China
with high/low flavonoids contents, respectively. CHI1 and CHI-31 were successfully expressed and the HPLC results indicated that the catalytical activities of CHI-1 were higher than that of CHI-31. This study analyzed the gene polymorphism of CHI and determined that CHI-1 was conducive to flavonoid biosynthesis. The results of this study will provide a basis for clarifying the function of CHI and further molecular breeding of the high-quality licorice. Keywords Chalcone isomerase Licorice Flavonoid Gene polymorphism Pichia pastoris GS115 Abbreviations CHI Chalcone isomerase Amp Ampicillin G418 Geneticin MD Minimal dextrise medium MM Minimal medium YPD Yeast peptone dextrose medium BMGY Buffered glycerol-complex medium BMMY Buffered methanol-complex medium HPLC High-performance liquid chromatography NCBI National center for biotechnology information CHR Chalcone reductase GT Glycosyl transferase CNVs Copy number variations SNP Single nucleotide polymorphism
123
Genet Resour Crop Evol
Introduction Licorice (Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza glabra L.) has been used in traditional Chinese medicine for more than two thousand years. According to Chinese pharmacopoeia, three original plants, Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza gl
Data Loading...
