Gene UCHL1 expresses specifically in mouse uterine decidual cells in response to estrogen
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ORIGINAL PAPER
Gene UCHL1 expresses specifically in mouse uterine decidual cells in response to estrogen Lishuang Hao1,2,3 · Di Song1 · Mengfei Zhuang2 · Yan Shi1 · Lin Yu1 · Yaping He1 · Jian Wang1 · Tingting Zhang2 · Zhaogui Sun1 Accepted: 16 April 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract UCHL1 is expressed specifically in the brain and gonads of almost all studied model organisms including Drosophila, zebrafish, amphibians, and mammals, suggesting a high degree of evolutionary conservation in its structure and function. Although UCHL1 has been involved in spermatogenesis in mice, its specific expression in mammal placenta remains elusive. Our previous work has revealed that UCHL1 is highly expressed in oocytes, and has been involved in mouse ovarian follicular development. Here, we further examined UCHL1 expression change in endometria during early natural pregnancy, with different stages of the estrous cycle and pseudopregnancy as control. The UCHL1 gene deletion model showed that UCHL1 protein is associated with endometrial development, and its deletion leads to infertility. Notably, we demonstrate evidence showing the distinct expression pattern of UCHL1: weak expression over the uterine endometria, strong expression in decidualized stromal cells at the implantation site with a peak at pregnancy D6, and a shift with primary decidualization to secondary decidualized zones. Using the delayed implantation, the delayed implantation activation, and the artificial decidualization models, we have demonstrated that strong expression of UCHL1 occurred in response to decidualization and estrogen stimulation. These observations suggest that during the early proliferation and differentiation of mouse uterine decidua, UCHL1 expression is up-regulated, and formed an unique intracellular distribution mode. Therefore, we proposed that UCHL1 is involved in decidualization, and possibly in response to estrogen regulation. Keywords UCHL1 · Estrogen · Embryo implantation · Decidualization · Mouse
Lishuang Hao and Di Song are co-first authors and contributed equally to this work. * Tingting Zhang [email protected]
Yaping He [email protected]
* Zhaogui Sun [email protected]
Jian Wang [email protected]
Lishuang Hao [email protected]
1
NHC Key Lab of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Medical School, Fudan University, Shanghai 200032, People’s Republic of China
2
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, People’s Republic of China
3
Shanghai TCM‑Integrated Hospital, Shanghai University of TCM, Shanghai 200082, China
Di Song [email protected] Mengfei Zhuang [email protected] Yan Shi [email protected] Lin Yu [email protected]
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Introduction Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1, previously known as protein gene product 9.5 PGP9.5) is an important member of ubiquitin C-terminal hydr
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