Genes and Proteins Underlying Microbial Urinary Tract Virulence Basi
Proceedings of the FEMS Symposium on Genes and Proteins Underlying Microbial Urinary Tract Virulence: Basic Aspects and Applications, held September 16-19, 1999, in Pécs, Hungary. Urinary tract infections are among the most frequent diseases caused by mic
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ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY Editorial Board: NATHAN BACK, State University of New York at Buffalo IRUN R. COHEN, The Weizmann Institute of Science DAVID KRITCHEVSKY, Wistar Institute ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research RODOLFO PAOLETTI, University of Milan
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Genes and Proteins Underlying Microbial Urinary Tract Virulence Basic Aspects and Applications Edited by
Levente Emo″dy Tibor Pál University of Pécs Pécs, Hungary
and
Jörg Hacker Gabriele Blum-Oehler University of Würzburg Würzburg, Germany
Kluwer Academic Publishers New York, Boston, Dordrecht, London, Moscow
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0-306-46840-9 0-306-46455-1
.OXZHU$FDGHPLF3XEOLVKHUV 1HZ 105 E. coli cells per mL as the single pathogen were included in the study. The GVVPQ fimbria producing S. enteritidis 27655-3b, its curli negative derivative and the PCR control E. coli ETEC CG-98-K5 1-2 and S. aureus strains were from our strain collection.
3.2
ELISA
Parallel wells of ELISA plates (Linbro) containing 200 µL colonisation factor (CF) broth8 were inoculated with the test strains and incubated at 30 °C or 37 °C for 48 h. The curli positive S. enteritidis strain and its curli negative derivative were included in all plates as positive and negative controls. The ELISA reaction was carried out as described9 using a polyclonal antibody raised against the curli (SEF17) of S. enteritidis 276553b2. The reaction was considered positive (i.e. th
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