Genetic and epistatic effects for grain quality and yield of three grain-size QTLs identified in brewing rice ( Oryza sa
- PDF / 475,889 Bytes
- 12 Pages / 547.087 x 737.008 pts Page_size
- 60 Downloads / 186 Views
Genetic and epistatic effects for grain quality and yield of three grain-size QTLs identified in brewing rice (Oryza sativa L.). Satoshi Okada & Ken Iijima & Kiyosumi Hori & Masanori Yamasaki
Received: 27 May 2020 / Accepted: 17 August 2020 # Springer Nature B.V. 2020
Abstract Rice (Oryza sativa L.) in Japan is not only a food staple but also an important material for the Japanese alcoholic beverage, sake. The grain used in sake brewing has different characters from the cooking rice grain, including a large grain size and high white-core expression rate (WCE). Because large-sized grains often have a heavy grain weight and higher yield, this trait is also important for cooking rice. Chalky grains, such as white-core or white-belly grains, are not ideal as cooking rice. Here, we report that three grain-size quantitative trait loci (QTLs; qGL4-2, qGWh5, qGWh10), derived from the brewing cultivar, Yamadanishiki, affect grain shape, chalky grain rate, and yield, using near isogenic and pyramiding lines in the genetic background of the cooking cultivar, Koshihikari. First, these QTLs
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01166-0) contains supplementary material, which is available to authorized users. S. Okada Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama, Japan K. Iijima : K. Hori Institute of Crop Science, National Agriculture and Food Research Organization, Ibaraki, Japan M. Yamasaki (*) Food Resources Education and Research Center, Graduate School of Agricultural Science, Kobe University, Kasai, Hyogo 675-2103, Japan e-mail: [email protected]
influenced multiple components of grain shape, where epistatic effects were detected between qGL4-2 and qGWh5, for grain width and thickness, and between qGL4-2 and qGWh10, for grain length. Therefore, these QTLs may coordinate to control grain shape. Second, lines harboring qGWh5 or qGWh10 at the Yamadanishiki allele exhibited increased WCE, whereas lines with qGL4-2 and qGWh10 exhibited decreased white-belly grain rate (WBR). Thus, grain shape is associated with the occurrence of chalky grain, where the chalky type depends on the QTL. Finally, we used total panicle weight of plants as a simplified rice yield index, and a promising line pyramiding qGL4-2 and qGWh5 emerged. In conclusion, qGL4-2 would be useful for the breeding of cooking rice, to decrease WBR, while qGWh5 and qGWh10 were definitely more beneficial for that of brewing rice, to increase grain weight and WCE. Keywords Grain shape . QTL . Epistasis . Grain quality . Rice yield . Pyramiding line Abbreviations ANOVA Analyses of variance BWR Basal-white grain rate DTH Days to heading GL Grain length GT Grain thickness GWh Grain width MWR Milky white grain rate NIL Near isogenic line
88
PN PYL QTL SN TGW TPW WCE WBR
Page 2 of 12
Panicle number Pyramiding line Quantitative trait locus Spikelet number per panicle 1000-grain weight Total panicle weight per plant White-core expression rate
Data Loading...
