Genetic mapping and identification of new major loci for tolerance to low phosphorus stress in rice
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RESEARCH ARTICLE
Genetic mapping and identification of new major loci for tolerance to low phosphorus stress in rice Abdul Malik Solangi1 • Hira Khanzada1 • Ghulam Mustafa Wassan1 • Adnan Rasheed1 • Ayaz Ali Keerio2 • Majeeduddin Solangi2 • Saba Khanzada1,2 Muhammad Faheem2 • Jianmin Bian1 • Xiaohua Pan1 • Rui Cai Han1 • Xunfeng He1 • Ziming Wu1
•
Received: 17 August 2019 / Revised: 24 December 2019 / Accepted: 30 July 2020 Ó The Author(s) 2020
Abstract Phosphorus (P) is one of the essential macronutrients for rice. In this study, we used 120 rice backcross recombinant inbred lines (BRILs) derived from a cross indica cv. Changhui 891 and japonica cv. 02428. To elucidate the genetic control of P deficiency tolerance in rice, we have used high quality SNPs bin markers to identify some important loci underlying phosphorus deficiency. The bin map was generated which includes 3057 bins covering distance of 1266.5 cM with an average of 0.41 cM between markers. Based on this map, 50 loci, including four novel loci, qSL-3, qRL-11, qSDW-1, qRDW1 with phenotypic variance 23.26%, 12.06%, 9.89% associated with P deficiency-related seedling traits were
identified. No significant QTLs was found for root length under P?, shoot fresh weight P- and root length, shoot fresh weight for P?, P- and their ratio respectively. Root fresh weight, and root dry weight were strongly correlated to each other, and QTLs for these variables were located on the same chromosome 1 at the same region. Notably, 3 pleiotropic regions is the pioneer of our study, and these regions would facilitate map-based cloning to expedite the MAS selection for developing low phosphorous tolerant varieties. This study not only improves our knowledge about molecular processes associated with P deficiency, but also provides useful information to understand the genetic architecture of low phosphorous tolerance.
Abdul Malik Solangi, Hira Khanzada and Ghulam Mustafa Wassan have contributed equally to this work. & Ziming Wu [email protected]
Jianmin Bian [email protected]
Abdul Malik Solangi [email protected]
Xiaohua Pan [email protected]
Hira Khanzada [email protected]
Rui Cai Han [email protected]
Ghulam Mustafa Wassan [email protected]
Xunfeng He [email protected]
Adnan Rasheed [email protected]
1
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, People’s Republic of China
2
Faculty of Crop Production, Sindh Agriculture University Tando Jam, Sindh, Pakistan
Ayaz Ali Keerio [email protected] Majeeduddin Solangi [email protected] Saba Khanzada [email protected] Muhammad Faheem [email protected]
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Physiol Mol Biol Plants
Keywords Rice Backcross recombinant inbreed lines Single nucleotide polymorphism QTL analysis Seedling traits Low phosphorus Abbreviations BRILs Backcross recombinant inbreed lines QTLs Quantitative trait loci SNP Single nucleotide polymorphism cm Cent
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