Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress
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ORIGINAL RESEARCH PAPER
Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress in soybean Shaohui Yang
. Yue Feng . Yue Zhao . Jingping Bai . Jiehua Wang
Received: 28 February 2020 / Accepted: 12 July 2020 Ó Springer Nature B.V. 2020
Abstract Objective To enhance Pi absorption and utilization efficiency of soybean, a member of PHT1 gene family was isolated and characterized from E. salsugineum, which was a homologous gene of AtPHT1;4 and consequently designated as EsPHT1;4. Results Quantitative real-time PCR (qRT-PCR) analysis showed that the transcript level of EsPHT1;4 significantly increased both in roots and leaves of E. salsugineum under Pi deficient conditions. Furthermore, EsPHT1;4 was transferred to soybean cultivar ‘‘YD22’’ using an Agrobacterium-mediated cotyledonary-node transformation method. Overexpression of EsPHT1;4 in soybean not only promoted the increase of plant biomass and yield of transgenic plants upon low P stress, but also increased the accumulation and transportation of Pi from roots to leaves in the transgenic soybean lines. Conclusion EsPHT1;4 was critical for controlling the accumulation and translocation of Pi in plants, and can be subsequently used as an effective foreign gene
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10529-020-02968-0) contains supplementary material, which is available to authorized users. S. Yang (&) Y. Feng Y. Zhao J. Bai J. Wang School of Environmental Science and Engineering, Tianjin University, Nankai Area, Weijin Rd. 92, Tianjin 300072, China e-mail: [email protected]
for the improvement of P use efficiency of crops by genetic manipulation. Keywords Eutrema salsugineum Genetic transformation Phosphate transporter Phosphorus Soybean
Introduction Phosphorus (P) is an essential macronutrient for life, and serves as a major structural component of nucleic acids, phospholipids, and phosphorylated intermediates of energy metabolism (Schachtman et al. 1998). Thus, P is an integral part of many physiological and biochemical processes in plant cells including activation of protein and regulating key enzyme reactions in metabolic pathways (Marschner 1995; Misson et al. 2004; Nussaume et al. 2011; Timlin et al. 2017). In plants tissues, Pi concentration is generally (5–20 mM) (Raghothama 1999), while the availability of Pi in soil is low (2–10 lM) and difficult to meet the growth demand (Marschner 1995; Nussaume et al. 2011). Therefore, Pi uptake and utilization by plants play a vital role in the determination of crop yield. Numerous adaptive mechanisms in plants have been developed to cope with Pi starvation, such as root morphology and geometry (Shen et al. 2011; Mendes et al. 2014; Strock et al. 2018), excretion of organic
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chelators and acid phosphatase, and substantial distribution of Pi from older leaves to younger organs (Smith et al. 2003), altered a
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