Genetic mapping of the three-pistil gene Pis1 in an F 2 population derived from a synthetic hexaploid wheat using multip
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ORIGINAL PAPER
Genetic mapping of the three‑pistil gene Pis1 in an F2 population derived from a synthetic hexaploid wheat using multiple molecular marker systems Z. Y. Yu1 · Q. Luo1 · Z. S. Peng1,2 · S. H. Wei1 · Z. J. Yang1 · N. Yamamoto1 Received: 1 April 2020 / Accepted: 4 September 2020 © Akadémiai Kiadó Zrt. 2020
Abstract The wheat spike mutant ’three-pistil’ (TP) exhibits normal external morphology but produces two additional pistils per floret. Much attention has been paid to this natural mutation because elucidation of its genetic foundation potentially paves a way to increase the number of grains per spike for breeding. Our previous studies roughly mapped the causal locus of TP on chromosome 2D. In this study, we performed genetic mapping of the three-pistil gene Pis1 in an F 2 population derived from a cross of TP and synthetic hexaploid wheat of which chromosome 2D was from Aegilops tauschii. Segregation analysis in the F2 population confirmed the dominance of the Pis1 locus. Bulked segregant analysis with three DNA marker systems revealed a total of 18 markers genetically linked with Pis1. Successive genetic linkage analysis of the F2 individuals using the 18 markers identified two sequence-related amplified polymorphisms markers, Me5-eM33 and Me26-eM33, flanked the Pis1 locus at distances of 0.6 and 1.7 cM, respectively. Integration of the genetic linkage and previously discovered DNA markers associated with Pis1 revealed a potential location of the Pis1 locus between Me5-eM33 and a KASP marker M75. Thus, the constructed partial genetic linkage map is useful for the isolation of Pis1 and marker-assisted breeding in wheat. Keywords Three-pistil mutant · Pis1 · SSR · SRAP · STS · Wheat
Introduction Wheat (Triticum aestivum L.), which is a cereal crop widely grown all over the world, supplies approximately 20% of the food source for human beings. Wheat grains are indispensable for bread baking, noodles, and pastries and have Communicated by E. Khlestkina. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42976-020-00078-1) contains supplementary material, which is available to authorized users. * Z. J. Yang [email protected] * N. Yamamoto [email protected] 1
Key Laboratory of Southwest China Wildlife Resources Conservation (ministry of education), College of Life Science, China West Normal University, Nanchong, Sichuan, China
School of Agricultural Science, Xichang University, Xichang, Sichuan, China
2
contributed to our diverse food culture. Meanwhile, generally grain yield in wheat is quite lower than in other popular cereals corn and rice; for instance, the current averaged yield in China is approximately 5481 kg/ha (FAO 2017). Therefore, to increase grain yield is still one of the main goals of wheat breeding. In general, there are approximately 20 spikelets in each spike and around 11 florets per spikelet in common wheat (Shitsukawa et al. 2009), hence increasing the number of grains per spike is the key for improving the yield f
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