Genome-wide analysis of Hsp70 and Hsp100 gene families in Ziziphus jujuba
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ORIGINAL PAPER
Genome-wide analysis of Hsp70 and Hsp100 gene families in Ziziphus jujuba Kishor Prabhakar Panzade 1
&
Sonam S. Kale 2
&
Narendra R. Chavan 2 & Bhupal Hatzade 3
Received: 24 July 2020 / Revised: 31 October 2020 / Accepted: 4 November 2020 # Cell Stress Society International 2020
Abstract The Ziziphus species are naturally tolerant to a range of abiotic stresses. Therefore, it is expected that they are an enriched source of genes conferring stress tolerance. Heat shock proteins (Hsps) play a significant role in plants in imparting tolerance against abiotic stress conditions. To get an insight into potential Hsp function in Ziziphus, we performed a genome-wide analysis and expression study of Hsp70 and Hsp100 gene families in Ziziphus jujuba. We identified 21 and 6 genes of the ZjHsp70 and ZjHsp100 families, respectively. Physiochemical properties, chromosomal location, gene structure, motifs, and protein domain organization were analysed for structural and functional characterization. We identified the contribution of tandem and segmental gene duplications in expansions of ZjHsp70s and ZjHsp100s in Z. jujuba. Promoter analysis suggested that ZjHsp70s and ZjHsp100s perform diverse functions related to abiotic stress. Furthermore, expression analyses revealed that most of the Z. jujuba Hsp genes are differentially expressed in response to heat, drought, and salinity stress. Our analyses suggested ZjHsp70-3, ZjHsp70-5, ZjHsp70-6, ZjHsp70-16, ZjHsp70-17, ZjHsp70-20, ZjHsp100-1, ZjHsp100-2, and ZjHsp100-3 are potential candidates for further functional analysis and with regard to breeding new more resilient strains. The present analysis laid the foundation for understanding the molecular mechanism of Hsps70 and Hsp100 gene families regulating abiotic stress tolerance in Z. jujuba. Keywords Genome-wide analysis . Heat shock protein (Hsp) . Gene family . Expression analysis . qRT-PCR . Z. jujuba
Introduction In recent decades, abiotic stress has caused severe economic losses of crop production globally. Abiotic stress hampers the * Kishor Prabhakar Panzade [email protected] Sonam S. Kale [email protected] Narendra R. Chavan [email protected] Bhupal Hatzade [email protected]; [email protected] 1
Division of Molecular Biology and Biotechnology, Indian Agriculture Research Institute, New Delhi 110012, India
2
Department of Plant Biotechnology, MGM College of Agricultural Biotechnology, Aurangabad 431007, India
3
Department of Plant Biotechnology, Ajeet Seeds Pvt. Ltd., Aurangabad 431133, India
biochemical and physiological processes of crop plants, which negatively affects their growth and development, thus cumulatively affecting crop yield as well as food quality (Wahid 2007; Hasanuzzaman et al. 2013). The global climate model has proposed that temperatures will rise by 1.4 to 5.8 °C by the end of the twenty-first century due to the increase in atmospheric greenhouse gases (Planton et al. 2016). So far, the progressively changing climate has affected food secur
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