Genome-wide characterization of coding and non-coding RNAs in the ovary of honeybee workers and queens
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Apidologie * The Author(s), 2020 DOI: 10.1007/s13592-020-00760-7
Genome-wide characterization of coding and noncoding RNAs in the ovary of honeybee workers and queens Xiao CHEN1 , Wei SHI1,2 1
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China 2 Xiangshan Beigou No. 1, Haidian District, Beijing 100093, China Received 10 September 2019 – Revised 24 February 2020 – Accepted 30 March 2020
Abstract – Adult honeybee queens and workers drastically differ in ovary state and ovary size. However, this reproductive bias is only partially understood from the view of a single RNA type. In this study, we predicted 10,271 mRNAs, 7235 lncRNAs, 11,794 circRNAs, and 164 miRNAs in the ovary of honeybee workers through bioinformatics. Combining RNA sequencing data of honeybee virgin queens, 4385 mRNAs, 2390 lncRNAs, 5602 circRNAs, and 75 miRNAs were differentially expressed in workers compared with virgins. Compared with egglaying queens, 6536 mRNAs, 3130 lncRNAs, 5751 circRNAs, and 81 miRNAs were differentially expressed in workers. Further, functional annotation revealed that neural regulation was closely related to ovary state. Moreover, the potential interactions among circRNAs, miRNAs, lncRNAs, and mRNAs revealed that vitellogenin, ecdysoneinduced protein 74, ame_circ_0001176, and ame_circ_0001243 might play critical roles in the competing endogenous RNA network. These findings suggest that the integrative RNA networks have potential effects in ovarian phenotype differences in honeybees. Apis mellifera ligustica / ovary / caste difference / coding RNA / non-coding RNA
1. INTRODUCTION Honeybee (Apis mellifera ligustica ) female ontogenies are governed by identical genomes (Macedo et al. 2016). However, differences in the quality of the food offered to young larvae trigger specific developmental pathways, producing highly specialized female phenotypes: queens and workers (Macedo et al. 2016). Adult honeybee queens and workers drastically differ in ovary size and ovarian state. Honeybee queens are equipped with huge ovaries (150–200 ovarioles). And after mating, the queen’s ovaries become activated and even bigger. Then, she initiates egg Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13592-020-00760-7) contains supplementary material, which is available to authorized users.
Manuscript editor: Klaus Hartfelder
laying. By contrast, the worker bees start with queen like ovaries and during development degeneration occurs leading to the worker ovary phenotype, i.e., small ovaries (1–20 ovarioles) and non-activated ovaries (Graham et al. 2011). The queen is capable of laying over 1000 eggs per day, whereas workers typically do not lay eggs in the presence of a queen (Winston 1987). This adult ovarian phenotype difference between queens and workers is due to different developmental pathways (Ashby et al. 2016; Duncan et al. 2016; Macedo et al. 2016). Characterizing castespecifically expressed genes in ovaries of honeybees has advanced the knowl
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