Genomic analyses of heat stress transcription factors (HSFs) in simulated drought stress response and storage root deter
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ORIGINAL ARTICLE
Genomic analyses of heat stress transcription factors (HSFs) in simulated drought stress response and storage root deterioration after harvest in cassava Jian Zeng1,5 · Chunlai Wu2 · Cheng Wang4 · Fengfeng Liao1 · Jiajia Mo1 · Zehong Ding2,3 · Weiwei Tie2,3 · Yan Yan2,3 · Wei Hu2,3 Received: 30 March 2020 / Accepted: 17 July 2020 © Springer Nature B.V. 2020
Abstract Heat shock factors (HSFs) play crucial roles in various plant stress responses. However, the current knowledge about HSFs in cassava, an important crop, is still insufficient. In this research, we identified 32 cassava HSF genes (MeHSFs) and clustered them into three groups (A, B, C) based on phylogenetic analysis and structural characteristics. Conserved motif analyses showed that MeHSFs display domains characteristic to HSF transcription factors. Gene structure analyses suggested that 29 MeHSFs contained only two exons. All identified 32 cassava MeHSFs were distributed on 13 chromosomes. Their expression profiles revealed that the different MeHSFs were expressed differentially in different tissues, most high expression genes belonged to group A. The similar MeHSFs were up-regulated after treatment with both PEG and abscisic acid (ABA), which implied that these MeHSFs may participate in resistance to simulated drought stress associated with the ABA signaling pathway. In addition, several MeHSFs were induced during postharvest physiological deterioration (PPD) in cassava. Our results provided basic but important knowledge for future gene function analysis of MeHSFs toward efforts in improving tolerance to abiotic stress and PPD in cassava. Keywords Cassava · Genome-wide · HSFs · Stress · Postharvest physiological deterioration
Introduction
Jian Zeng, Chunlai Wu, Cheng Wang have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05673-3) contains supplementary material, which is available to authorized users. * Wei Hu [email protected] 1
Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan, China
Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‑Season Reproduction Regions, Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
2
Plants routinely experience diverse stress conditions, such as abiotic or biotic stresses, due to their sessile nature. They are therefore required to adapt for survival by developing a range of defense mechanisms [1, 2]. Heat shock response is an important conserved defense mechanism for adapting to environmental stresses. Heat shock proteins (HSPs) play essential roles in defense from environment stresses by folding/unfolding and degrading proteins [3, 4]. Several 3
Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropic
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