Genomic comparison of Clostridium chauvoei isolates from classical and visceral clinical manifestation
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VETERINARY MICROBIOLOGY - RESEARCH PAPER
Genomic comparison of Clostridium chauvoei isolates from classical and visceral clinical manifestation Rosangela Estel Ziech 1 & Franciele Maboni Siqueira 2 & Samuel Cibulski 3 & Silvia De Carli 4 & Helton Fernandes dos Santos 5 & Rafael Almeida Fighera 5 & Joachim Frey 6 & Agueda Castagna de Vargas 1 Received: 5 October 2018 / Accepted: 28 June 2019 # Sociedade Brasileira de Microbiologia 2019
Abstract Clostridium chauvoei is the etiological agent of blackleg, an infectious disease affecting cattle and small ruminants worldwide. This disease can manifest as classical blackleg, a condition in which skeletal muscles are affected and visceral blackleg, which affects the heart, sublingual muscles, and the diaphragm. The pathogenesis of the visceral form of the disease is poorly understood. The objective of this study is to determine and analyze complete genomic sequences of six C. chauvoei strains, five isolates from skeletal muscle and one isolate from a visceral case of blackleg in Brazil, to provide insights into the differences in pathogenic profiles of strains causing the different forms of disease. The full genomes of the six C. chauvoei strains were sequenced and comparative analyses were performed among these genomes and the C. chauvoei reference strain JF4335. The results of this study revealed that the genomes of the C. chauvoei strains analyzed are highly conserved; no particular differences were noted that could be associated with the two different clinical manifestations of the disease. Keywords Blackleg . Clinical manifestations . Genomic analysis
Introduction Clostridium chauvoei is a Gram-positive, anaerobic, sporeforming rod found in the soil, feces, and the digestive tract Responsible Editor: Agnes Figueiredo. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42770-019-00177-0) contains supplementary material, which is available to authorized users. * Rosangela Estel Ziech [email protected] 1
Laboratório de Bacteriologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
2
Laboratório de Bacteriologia Veterinária, Departamento de Patologia Clínica Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
3
Laboratório de Virologia, UFRGS, Porto Alegre, RS, Brazil
4
Laboratório de Diagnóstico Molecular, Universidade Luterana do Brasil, Canoas, RS, Brazil
5
Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
6
Vetsuisse Faculty, University of Bern, Bern, Switzerland
of many animals [1]. Transmission of blackleg occurs through the ingestion of C. chauvoei spores from contaminated soil. The ingested spores, or those produced after germinative cycles in the gut, are transported from the intestine or lesions in the oral cavity to muscles and tissues by macrophages across Peyer’s patches [2, 3]. Once localized in the tissues, the spores remain dormant until specific conditions such as anaerobiosis lead to thei
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