Growth Phase-Dependent Synthesis of Gold Nanoparticles Using Bacillus Licheniformis
Biological agents play an important role in biosynthesis of nanoparticles and regarded as green technology and environmental-friendly approach, which is an important step in the field of application of nanotechnology. The present study reports biosynthesi
- PDF / 232,160 Bytes
- 8 Pages / 439.37 x 666.142 pts Page_size
- 52 Downloads / 232 Views
Abstract
Biological agents play an important role in biosynthesis of nanoparticles and regarded as green technology and environmental-friendly approach, which is an important step in the field of application of nanotechnology. The present study reports biosynthesis of gold nanoparticles from gold precursor using Bacillus licheniformis at 37 °C depending upon its growth phase. Growth phase study of bacteria was performed and biosynthesis of gold nanoparticles was carried out at early log phase, mid log phase, stationary phase, and decline phase. The synthesis of gold nanoparticles was confirmed by UV–visible spectrum that showed peak between 545 and 559 nm which was later reconfirmed by SEM studies. Size of gold nanoparticles as measured by dynamic light scattering was found to be in the range of 16–95 nm. Zeta potential measurement showed that gold nanoparticles were negatively charged with zeta potential value between −20.8 and −25.8 mV. The stationary phase culture, out of early and late log phase showed best results, synthesizing small-sized gold nanoparticles. Keywords
Bacillus licheniformis Biosynthesis Gold nanoparticles Growth phase Zeta potential
S. Tikariha S. Banerjee S. Singh (&) Department of Bio-Engineering, BIT, Mesra, Ranchi, Jharkhand, India e-mail: [email protected] A. Dev Department of Pharmaceutical Sciences & Technology, BIT, Mesra, Ranchi, Jharkhand, India © Springer Nature Singapore Pte Ltd. 2017 K. Mukhopadhyay et al. (eds.), Applications of Biotechnology for Sustainable Development, DOI 10.1007/978-981-10-5538-6_15
121
122
S. Tikariha et al.
Introduction Recently, the synthesis of nanogold has become increasingly important owing to their unique properties that differ from their bulk counterparts (Armendariz et al. 2004). The nanosized gold particles find applications in diverse fields as catalysis, sensors and medicine which largely depend on the size and composition of the nanoparticles (Haverkamp et al. 2007). Synthesis of nanoparticles can be achieved through chemical, physical, and biological methods. As nanogold is widely used in vivo in human especially in contacting areas, there is a growing need to develop environmentally benign processes for their synthesis. As a result, biological approach of nanoparticles synthesis by using different microorganism, enzyme, and plant extract have emerged as possible alternatives to synthetic methods (Song and Kim 2009). A wide variety of microorganism has been used as a biofactory for biosynthesis of gold nanoparticles. Handling of bacteria is easy and they can be easily manipulated as per requirement, so bacterial system has gained importance for the synthesis of gold nanoparticles (Sweeney et al. 2006). The present study reports a simple biological synthesis of gold nanoparticles through Bacillus licheniformis by reduction of chloroaurate ions depending upon its different growth phase study. This work is the comprehensive kinetic study of gold nanoparticles aggregation that can be used to predict the appropriate growth
Data Loading...
