Gut microbiota characteristics in mice with antibiotic-associated diarrhea
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RESEARCH ARTICLE
Open Access
Gut microbiota characteristics in mice with antibiotic-associated diarrhea Haoqing Shao1,2, Chenyang Zhang1,2, Nenqun Xiao3 and Zhoujin Tan2,4*
Abstract Background: Antibiotic-associated diarrhea (AAD), defined as diarrhea that occurs in association with the administration of antibiotics and without another clear etiology, is one of the most common adverse drug events of antibiotics therapy. We established a diarrhea model induced by gentamycin and cefradine to investigate the microbiota characteristics in the intestinal lumen of mice with AAD and provide insights into noteworthy bacteria related to gentamicin and cefradine-associated diarrhea. Results: The number of OTUs in the model group and the normal group was 983 and 2107, respectively, and 872 identical OTUs were shared between two groups. Species richness and species diversity of intestinal microbe were altered by antibiotics administration. PCoA showed a clear separation between AAD and health control. The dominant phyla of AAD mice were Firmicutes (52.63%) and Proteobacteria (46.37%). Among the genus with top 20 abundance, the relative abundance of 7 genera, Ruminococcus, Blautia, Enterococcus, Eubacterium, Clostridium, Coprococcus, and Aerococcus, were enriched in the model group. Based upon the LEfSe analysis, Enterococcus, Eubacterium, Ruminococcus, and Blautia were identified as potential biomarkers for AAD. Conclusions: The bacterial diversity of the intestinal lumen was diminished after gentamicin and cefradine administration. The alterations in the abundance and composition of gut microbiota further led to the dysfunction of gut microbiota. More specifically, gentamicin and cefradine significantly increased the abundance of the opportunistic pathogens, of which Enterococcus and Clostridium were the most prominent and most worthy of attention. Keywords: Gut microbiota, Antibiotic-associated diarrhea, Gentamicin, Cefradine, Enterococcus, Clostridium, 16S rRNA gene sequencing
Background Antibiotics are frequently prescribed drugs for clinical treatment of various bacterial infections. Yet several adverse drug events (ADE) have emerged as the widespread use of antibiotics. Antibiotic-associated diarrhea (AAD), defined as diarrhea that occurs in association with the administration of antibiotics and without * Correspondence: [email protected] 2 Hunan Key Laboratory of TCM Prescription and Syndromes Translational Medicine, Changsha, Hunan, China 4 School of Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China Full list of author information is available at the end of the article
another clear etiology, is one of the most common ADE of antibiotics administration [1]. AAD is a complex disease that is affected by the host, infectious agent involved, and numerous clinical elements, including antibiotics therapeutic scheme. AAD can affect up to a third of the patients receiving a particular antibiotic [2], but the incidence and severity of AAD vary among different antibiotics. We observed more serious diarrh
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