High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid
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BioMed Central
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Methodology
High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia Gero Hütter*1, Anne Letsch1, Daniel Nowak1, Julia Poland2, Pranav Sinha2, Eckhard Thiel1 and Wolf-K Hofmann1 Address: 1Department of Internal Medicine III (Hematology, Onkology), Charité Berlin Campus Benjamin Franklin, Berlin, Germany and 2Institute of Laboratory Medicine and Clinical Chemistry, LKH Klagenfurt, Austria Email: Gero Hütter* - [email protected]; Anne Letsch - [email protected]; Daniel Nowak - [email protected]; Julia Poland - [email protected]; Pranav Sinha - [email protected]; Eckhard Thiel - [email protected]; WolfK Hofmann - [email protected] * Corresponding author
Published: 15 January 2009 Journal of Translational Medicine 2009, 7:7
doi:10.1186/1479-5876-7-7
Received: 12 September 2008 Accepted: 15 January 2009
This article is available from: http://www.translational-medicine.com/content/7/1/7 © 2009 Hütter et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens. However, it remains to be determined, whether this correlation also exists on the level of protein expression. Method: Therefore, we investigated both bone marrow and peripheral blood blast cells from six patients with newly diagnosed acute myeloid leukemia (AML) using conventional two-dimensional electrophoresis in the first dimension and linear polyacrylamide gels (12%) in the second dimension. Proteins were visualized using the silver staining method and image analysis was performed using the PDQuest system. Results: For each patient over 80 proteins were evaluated in the sample from peripheral blood and bone marrow. We could demonstrate that the protein expression profile of bone marrow did not significantly differ from the expression patterns of peripheral blast cells. Conclusion: The proteome-set of leukemic blast cells from marrow and blood, does not differ substantially when drawn from AML patients with over 80 percent blast cells in both compartments. This indicates that in AML, blasts from peripheral blood samples can be considered suitable for investigations of the proteome using 2D-electrophoresis.
Background Acute myeloid leukemia (AML) is an aggressive hematological neoplasia and it is characterized by accumulating myeloid precursor cells in bone marrow and detection of such cells in peripheral blood. Cytogenetics and molecular diagnostics are helpful for an individualized therapy in certain subsets of AML. There is hope that proteomics in AML will prompt new diagnostic or therapeutic biomark-
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