High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances a
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BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS
High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors Nina Jankowski 1 & Katja Koschorreck 1 & Vlada B. Urlacher 1 Received: 22 June 2020 / Revised: 14 August 2020 / Accepted: 2 September 2020 / Published online: 19 September 2020 # The Author(s) 2020
Abstract The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastics, bioactive compounds, and flavors and fragrances. Arylalcohol oxidase 2 (PeAAO2) from the fungus Pleurotus eryngii was heterologously expressed and secreted at one of the highest yields reported so far of 315 mg/l using the methylotrophic yeast Pichia pastoris (recently reclassified as Komagataella phaffii). The glycosylated PeAAO2 exhibited a high stability in a broad pH range between pH 3.0 and 9.0 and high thermal stability up to 55 °C. Substrate screening with 41 compounds revealed that PeAAO2 oxidized typical AAO substrates like p-anisyl alcohol, veratryl alcohol, and trans,trans-2,4-hexadienol with up to 8-fold higher activity than benzyl alcohol. Several compounds not yet reported as substrates for AAOs were oxidized by PeAAO2 as well. Among them, cumic alcohol and piperonyl alcohol were oxidized to cuminaldehyde and piperonal with high catalytic efficiencies of 84.1 and 600.2 mM−1 s−1, respectively. While the fragrance and flavor compound piperonal also serves as starting material for agrochemical and pharmaceutical building blocks, various positive health effects have been attributed to cuminaldehyde including anticancer, antidiabetic, and neuroprotective effects. PeAAO2 is thus a promising biocatalyst for biotechnological applications. Key points • Aryl-alcohol oxidase PeAAO2 from P. eryngii was produced in P. pastoris at 315 mg/l. • Purified enzyme exhibited stability over a broad pH and temperature range. • Oxidation products cuminaldehyde and piperonal are of biotechnological interest. Keywords Aryl-alcohol oxidase . Pichia pastoris (Komagataella phaffii) . Flavoprotein . Aromatic alcohols . Fragrances . Piperonal
Introduction The pursuit of a sustainable and bio-based society includes the search for and development of environmentally friendly production routes for fine chemicals. As a result, more and more Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10878-4) contains supplementary material, which is available to authorized users. * Vlada B. Urlacher [email protected] 1
Institute of Biochemistry, Heinrich-Heine-University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany
biocatalytic processes for production of fine chemicals and valuable building blocks are coming into the focus of research and industry. I
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