Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high-fat diet
- PDF / 1,633,614 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 73 Downloads / 198 Views
ORIGINAL CONTRIBUTION
Effect of mushroom polysaccharides from Pleurotus eryngii on obesity and gut microbiota in mice fed a high‑fat diet Daiki Nakahara1 · Cui Nan1 · Koichiro Mori2 · Motoki Hanayama2 · Haruhisa Kikuchi3 · Shizuka Hirai1 · Yukari Egashira1 Received: 17 July 2019 / Accepted: 9 December 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract Purpose Mushrooms are reported to have a variety of health-promoting activities. However, little information is available on the effects of intake of polysaccharides from Pleurotus eryngii on obesity. In this study, we investigated the effects of P. eryngii polysaccharides on obesity and gut microbiota in mice fed a high-fat diet. Methods Soluble polysaccharides were extracted from P. eryngii using hot water. C57BL/6J mice were fed a standard diet (ST), a high-fat diet (HF), or HF with 1% or 5% P. eryngii polysaccharide fraction (LP or HP) for 16 weeks. Adipose tissues were weighed and blood parameters were measured. Expression of genes involved in fatty acid and cholesterol metabolism was assessed by real-time quantitative PCR. The gut microbiota composition was analysed by 16S rRNA gene sequencing. Results Body weight gain and mesenteric fat tissue were lower in the HP group than in the HF group. In the HP group, serum total cholesterol and LDL cholesterol levels decreased, and lipid and total bile acids in faeces increased. Mice in the HP group showed increased expression of the LDLR gene in the liver and GPR43 in fat. The relative abundance of Firmicutes was significantly higher in the HF and HP groups than in the ST group. The abundance of some short-chain fatty acid-producing gut bacteria was altered by P. eryngii polysaccharides. Conclusions These results provide the first evidence that P. eryngii polysaccharides have anti-obesity and LDL cholesterollowering effects in obese mice through increased excretion of bile acids and lipids and altered microbiota. Keywords Pleurotus eryngii · Mushroom · Obesity · Gut microbiota · LDL cholesterol · GPR43 · Dietary fibre · Polysaccharide
Introduction In recent years, obesity has become an increasing problem worldwide [1]. Obesity and related metabolic disorders such as hypertension, dyslipidaemia, and glucose intolerance cause arterial sclerosis, which is a major cardiovascular risk factor. Therefore, it is important to prevent or ameliorate * Yukari Egashira [email protected]‑u.jp 1
Laboratory of Food and Nutrition, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271‑8510, Japan
2
Mushroom Research Laboratory, Hokuto Corporation, 800‑8 Shimokomazawa, Nagano 381‑0008, Japan
3
Laboratory of Natural Products Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, 6‑3 Aoba, Aramaki, Aoba‑ku, Sendai 980‑8578, Japan
obesity. There are many approaches to manage obesity such as diet modification, exercise, pharmacotherapy, and bariatric surgery [2]. Diet modification is the simplest method among them and has fewer side effects t
Data Loading...
