High Productivity Ethanol from Solid-State Fermentation of Steam-Exploded Corn Stover Using Zymomonas mobilis by N 2 Per
- PDF / 2,458,181 Bytes
- 16 Pages / 439.37 x 666.142 pts Page_size
- 24 Downloads / 184 Views
High Productivity Ethanol from Solid-State Fermentation of Steam-Exploded Corn Stover Using Zymomonas mobilis by N2 Periodic Pulsation Process Intensification Lele Sun 1,2 & Lan Wang 1
& Hongzhang Chen
1
Received: 11 February 2020 / Accepted: 23 April 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solidstate fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol fermentation. Z. mobilis can produce ethanol from glucose near the theoretical maximum value, but this ethanol yield would be greatly decreased by high oxygen partial pressure during solid-state fermentation. In this study, we applied N2 periodic pulsation process intensification (NPPPI) to ethanol solid-state fermentation, which displaced air with N2 and provided a proper anaerobic environment for Z. mobilis. Based on the water state distribution, the promotion effects of NPPPI on low solid loading and solid-state fermentation were analyzed to confirm the different degrees of oxygen inhibition in ethanol solid-state fermentation. During the simultaneous saccharification solid-state fermentation, the NPPPI group achieved 45.29% ethanol yield improvement and 30.38% concentration improvement compared with the control group. NPPPI also effectively decreased 58.47% of glycerol and 84.24% of acetic acid production and increased the biomass of Z. mobilis. By coupling the peristaltic enzymatic hydrolysis and fed-batch culture, NPPPI made the ethanol yield and concentration reach 80.11% and 55.06 g/L, respectively, in solid-state fermentation. Keywords Lignocellulosic ethanol . Solid-state fermentation . N2 periodic pulsation process intensification . Zymomonas mobilis . Simultaneous saccharification fermentation . Periodic peristalsis . Steam explosion
* Lan Wang [email protected]
1
State Key Laboratory of Biochemical Engineering, Beijing Key Laboratory of Biomass Refining Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China
2
University of Chinese Academy of Sciences, Beijing 100190, People’s Republic of China
Applied Biochemistry and Biotechnology
Introduction Increasing depletion of fossil energy and its negative impacts on the environment, society was forced to look for renewable energy (1). Bioethanol, as promising and sustainable fuel, has advantages of pollution-free, abundant reserves, and renewable characteristics (1). Corn stover is a cheap agricultural residue and rich in cellulose, which is an important resource for the production of bioethanol (2). However, it is difficult to directly convert cellulose into glucose due to the inherent existence of the biomass recalcitrance (3). In order to break the biomass recalcitrance and improve the efficiency of ethanol conversion, pretreatment is particularl
Data Loading...
