Highly Sensitive and Fast Detection of C-Reactive Protein and Troponin Biomarkers Using Liquidgated Single Silicon Nanow
- PDF / 1,335,745 Bytes
- 12 Pages / 432 x 648 pts Page_size
- 42 Downloads / 192 Views
MRS Advances © 2020 Materials Research Society DOI: 10.1557/adv.2020.60
Highly Sensitive and Fast Detection of C-Reactive Protein and Troponin Biomarkers Using Liquidgated Single Silicon Nanowire Biosensors Yurii Kutovyi 1, λ, Jie Li 1, λ, Ihor Zadorozhnyi 1, Hanna Hlukhova 1, Nazarii Boichuk 1, Dmytro Yehorov 1, Marcus Menger 2 and Svetlana Vitusevich 1* 1
Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany
2
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), 14476 Potsdam, Germany
λ
These authors contributed equally to the work.
*
E-mail: [email protected]
ABSTRACT
C-reactive protein (CRP) and cardiac troponin I (cTnI) biomolecules represent the earliest enzymes that appear in the blood when a cardiac injury occurs. Real-time and selective detection of these biomarkers is essential for the prediction and detection of cardiovascular diseases at an early stage. Here we report on the label-free specific detection of both proteins at picomolar concentrations using fabricated nanowire-based biosensors. We demonstrate a novel functionalization technique based on the attachment of dibenzocyclooctyne (DBCO)linked troponin-specific aptamers to azide-functionalized silicon (Si) nanowire (NW) surface. Due to the fast and reliable immobilization of cTnI-specific aptamers and CRP-specific antibodies on the Si NWs, the fabricated devices can rapidly detect target biomolecules demonstrating high sensitivity. We confirm the attachment of proteins to the surface of Si NWs by atomic force microscopy (AFM). Moreover, we demonstrate that nanowire structures of different sizes enable the detection of biomarkers in a wide concentration range (from 1 pg/ml to 1 µg/ml), corresponding to CRP and cTnI elevation levels during the early stage of disease formation.
Downloaded from https://www.cambridge.org/core. The Librarian-Seeley Historical Library, on 04 Feb 2020 at 05:21:03, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.1557/adv.2020.60
INTRODUCTION To predict and prevent disease development it is important to monitor the increased concentration of biological markers associated with a certain disease [1–3]. It has recently been established that the concentration levels of such proteins as C-reactive protein (CRP) and cardiac troponin I (cTnI) rise rapidly in the blood as a consequence of damage to the heart muscle [3–5]. Therefore, the development of a detection system allowing the highly sensitive, fast and selective detection of both biomarkers is essential in the struggle against cardiovascular diseases [3]. Several techniques have already been proposed for sensing the aforementioned biomolecules [5–10]. The most common approach is an enzyme-linked immunosorbent assay (ELISA). However, the requirement for expensive and bulky laboratory equipment along with the involvement of a welltrained operator are the main drawbacks of ELISA for real medical applications [10,11]. In this r
Data Loading...
