Highly Specific Anti-tylosin Monoclonal Antibody and Its Application in the Quantum Dot Bead-Based Immunochromatographic
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Highly Specific Anti-tylosin Monoclonal Antibody and Its Application in the Quantum Dot Bead-Based Immunochromatographic Assay Xiaocui Lai 1 & Xi Lv 1 & Ganggang Zhang 1 & Zhijuan Xiong 1 & Weihua Lai 1 & Juan Peng 1 Received: 3 April 2020 / Accepted: 25 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this study, mAb-5A7 and mAb-1D7, two highly specific monoclonal antibodies against tylosin (TYL), were prepared by using hapten TYL-carboxymethoxylamine hemihydrochloride (TYL-CMO), and they showed IC50 values of 13.84 ng/mL and 13.01 ng/mL and LODs of 1.21 and 0.98 ng/mL, respectively, in indirect competitive enzyme-linked immunosorbent assay. An immunochromatographic assay based on quantum dot (QD)-embedded nanobead (QB-ICA) and mAb-1D7 was developed for the detection of TYL in milk and honey. The IC50 values of QB-ICA in PBS, milk, and honey were calculated to be 22.4, 18.5, and 24.41 ng/mL, with LODs of 2.32, 1.84, and 2.69 ng/mL, respectively, satisfying the requirement of the maximum residue limit for TYL in food samples of 100–300 μg/kg in China. The cross-reactivity (CR) values with two related compounds, tilmicosin and abamectin, were 4.48% and 1.87%, respectively, and the CR values with other drugs were all less than 0.2%, which indicated that the developed QB-ICA was highly specific for the determination of TYL. In addition, recovery experiments indicated that the recoveries ranged from 92.16 to 108.5% in milk and from 84.12 to 110.5% in honey. Therefore, the developed QB-ICA is reliable for determining TYL in milk and honey. In conclusion, the QB-ICA we developed can be considered an effective and rapid tool for screening TYL in food products. Keywords Specific monoclonal antibody . Tylosin . Immunochromatographic assay . Quantum dot nanobead
Introduction Tylosin (TYL) is widely used as a veterinary medicine to promote growth and prevent respiratory and enteric infections in swine, cattle, sheep, and poultry because of its broad applications and low toxicity (Galarini et al. 2015; Peng et al. 2012). However, TYL has a low metabolic rate after administration, so abuse of TYL could lead to considerable remnants in products and environmental pollution that would threaten human health and lead to drug resistance (Thompson et al. 2003; Yao et al. 2018). Therefore, the European Union prohibits the use of TYL in feed additives. In China, the
Xiaocui Lai and Xi Lv contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12161-020-01846-9) contains supplementary material, which is available to authorized users. * Juan Peng [email protected] 1
State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China
maximum residue limit (MRL) for TYL has been established as 100 to 300 μg/kg in food samples (GB31650-2019). Immunoassays have emerged as versatile complementary analytical tools to conventional instrumental analyses for onsite test
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