Silica-Based Gold Nanoshells: Synthesis and Application in Immunochromatographic Assay
- PDF / 697,781 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 80 Downloads / 229 Views
ca-Based Gold Nanoshells: Synthesis and Application in Immunochromatographic Assay V. G. Grigorenkoa, *, I. P. Andreevaa, G. V. Presnovaa, D. E. Presnovb, c, E. A. Yakovlevaa, and A. P. Osipova aDepartment
of Chemistry, Moscow State University, Moscow, 119991 Russia Skobeltsyn Institute of Nuclear Physics, Moscow State University, Moscow, 119991 Russia c Department of Physics, Quantum Technology Centre, Moscow State University, Moscow, 119991 Russia *e-mail: [email protected] b
Received February 10, 2020; revised February 12, 2020; accepted February 20, 2020
Abstract—We optimize the procedure for synthesizing gold nanoshells for their use as a sensitive label in an immunochromatographic assay (ICA). An immunochromatographic test system is developed to visually determine the concentration of an early cardio marker, a heart-type fatty acid-binding protein (H-FABP). Visual detection yields the detection limit of the procedure with gold nanoshells to be more 50% lower than that of gold nanoparticles; it is 0.5 ng/mL for H-FABP. Keywords: immunochromatographic assay, gold nanoshells, gold nanoparticles, fatty acid-binding protein DOI: 10.3103/S0027131420040021
Immunochromatographic assay (ICA) or lateral flow immunoassay (LFIA) is widely used for the rapid detection of a variety of analytes and the diagnosis of many diseases [1]. This analysis enables visual determination and evaluation of the concentration of various antigens, antibodies, hormones, and other diagnostically core substances in the body within a few minutes. A complex of biomolecules with various labels (gold nanoparticles, magnetic particles, quantum dots, nonspherical and inhomogeneous particles, luminescent and fluorescent labels, etc.) is used as a detecting agent for ICA [2–4]. Gold nanoparticles (GNPs) are rather popular as visual detecting agents because they can be easily obtained in the given size and have a high level of detection sensitivity [5–7]. Gold nanoparticles are more stable and easier to use than, for example, fluorescent or enzymatic labels. However, the required sensitivity of the analysis may be unattainable using gold nanoparticles; in this case, various methods are used to improve sensitivity, for example, enhancement by silver [8] or an increase in the size of gold particles [9]. However, obtaining stable and homogeneous gold nanoparticles with a diameter of more than 50 nm is a rather difficult task [10]. Gold nanoshells on silicon dioxide nuclei can serve as markers in immunochromatography [11, 12]. Nanoshells are nanocomposite spherical materials, in which particles of one material are coated with a thin layer of another material. Nanoshells can be synthesized from virtually any material: semiconductors,
metals, and dielectrics. Silicon dioxide is widely used as a core in a nanoshell due to its resistance to coagulation and chemical inertness [11]. The main advantage of nanoshells on conventional colloidal gold particles lies in their unique optical properties accompanied by particle stability. The band of the pla
Data Loading...
