HPLC Assay of Rutin Content in the Leaves of the Black Poplar
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Pharmaceutical Chemistry Journal, Vol. 54, No. 7, October, 2020 (Russian Original Vol. 54, No. 7, July, 2020)
MEDICINAL PLANTS HPLC ASSAY OF RUTIN CONTENT IN THE LEAVES OF THE BLACK POPLAR V. A. Kurkin1,* and E. A. Kupriyanova1 Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 54, No. 7, pp. 33 – 36, July, 2020.
Original article submitted August 1, 2019. A method for assay of rutin content in the leaves of the black poplar (Populus nigra L.) using HPLC at a wavelength of 360 nm was developed; this used an Elsico column (No. 10302, 2 mm ´ 80 mm; Silasorb C18 7 mm), with a mobile phase consisting of acetonitrile-water (2:8) supplemented with 1% glacial acetic acid. The suitability of the method for analysis of standard rutin samples was confirmed. Rutin content in black poplar leaves ranged from 0.67% to 1.05%. Statistical analysis of the experimental results provided evidence that the error level in single determinations of rutin in black poplar leaves with a 95% confidence interval was ± 4.6%. Keywords: black poplar, Populus nigra L., leaves, flavonoids, rutin, spectrophotometry, HPLC.
The search for potential medicinal plants to produce Russian-made drugs is an important aspect of contemporary pharmacy [1-7]. Drugs based on poplar buds have antibacterial, anti-inflammatory, antifungal, wound-healing, and actoprotective activities [2, 3, 5, 7-18]. In folk medicine, juice prepared from the leaves of the black poplar (Populus nigra L.) is used for dental pain and soothing baths, and as an antibacterial substance [19, 20]. Thus, along with black poplar buds, the leaves too are a potential source of biologically active compounds [5, 8, 20]. Published data indicate that black poplar leaves contain simple phenols (salicin, salicortin, nigracin, populin), phenylpropanoids (caffeic acid 3-O-b-D-glucopyranoside, lignans), tannins, vitamins (C, E), carotenoids (neoxanthin, violaxanthin, lutein), and organic acids - formic, oxalic, malonic, glyceric, succinic, fumaric, malic, tartaric, a-ketoglutaric, citric, and quinic [3, 20, 21]. Despite existing published data on the chemical composition of black poplar leaves [3, 5, 20 – 24], the literature lacks reports on the component composition of flavonoids in this material. We have previously developed a method for assay of total flavonoids in black poplar leaves using differential 1 *
spectrophotometry with a standard sample (reference material, RM) of rutin [25]. The aim of the present work was to develop a method for assay of rutin in black poplar leaves using HPLC. EXPERIMENTAL SECTION Studies were performed using black poplar leaves collected in June and September 2012-2018 in Alekseevka and Gavrilova Polyana, Samara district. Phytochemical studies of the medicinal herbal material (MHM) black poplar leaves
OH OH
2' 8
HO
O
6
O OH
O
6''
5'
O
6' 1''
O OH
2''
OH
OH H 3C HO
1'''
O OH OH
Samara State Medical University, 443099 Samara, Russia. e-mail: [email protected]
Fig. 1. Structural formula of rutin.
717 0091-150X/20/5407-0717 © 2020 Spring
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