Development of a Novel Derivate Assay for Formaldehyde Determination by HPLC in Beer Samples
- PDF / 534,567 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 36 Downloads / 227 Views
Development of a Novel Derivate Assay for Formaldehyde Determination by HPLC in Beer Samples Jingchan Zhao 1 & Gailing Wang 1 & Ting Cao 1 & Zhian Guo 1
Received: 29 January 2015 / Accepted: 17 April 2015 # Springer Science+Business Media New York 2015
Abstract In this paper, a novel derivative reagent, ethoxyamine hydrochloride (EAHC), was proposed for formaldehyde detection with a simple and highly sensitive pre-column derivation–high-performance liquid chromatography. The effects such as the concentration of derivative reagent, buffer solution pH, reaction temperature, and reaction time on the derivate reaction were also investigated. Under the optimized experimental conditions, the peak area showed a linear relationship with the concentration of formaldehyde in a range of 0.046 to 4.6 mg L−1, with a correlation coefficient of 0.9998. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.016 and 0.042 mg L−1, respectively. The proposed method was applied to the assay of different beer samples, and the recovery of the developed method was in the range of 91.3–104.3 %. To further evaluate the validity of the developed method, formaldehyde was also determined in different beer samples by the referenced HPLC method based on pre-column derivatization with 2, 4-dinitrophenylhydrazine (DNPH). Through the t test method, no obvious difference between the two methods was found at a 95 % confidence level. The results showed that the proposed method was a rapid, convenient, and feasible method for the determination of formaldehyde in beer samples.
* Jingchan Zhao [email protected] 1
Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, No. 229 North Taibai Road, Xi’an 710069, China
Keywords Formaldehyde . Derivative reagent . High-performance liquid chromatography . Ethoxyamine hydrochloride
Introduction During beer storage, beer quality is gradually deteriorated and the production of stale flavor, formation of haze, and browning occurs (Wang et al. 2012b). Consequently, formaldehyde was added to some beers to keep them pleasant (Cui et al. 2007; Zhao and Zhang 2009; Yue et al. 2007; Wang et al. 2012a; Lachenmeier et al. 2013). However, the International Agency for Research on Cancer has concluded that formaldehyde is Bprobably carcinogenic^ (group 2A), while in the meantime, formaldehyde was upgraded to group 1 by the IARC (IARC 2012). Nevertheless, the International Programme on Chemical Safety has established a tolerable concentration of 2.6 mg L−1 in ingested products. In fact, the concentration of formaldehyde was exceeded in some alcoholic beverages (Zhang et al. 2011; Jendral et al. 2011; Monakhova et al. 2012). Considering our human health, a rapid and simple method for the determination of formaldehyde in beer samples is required. However, it is very difficult to detect formaldehyde directly in complex matrices due to the lack of intrinsic chromophores or fluorophores as well as their
Data Loading...
