Human placental extract attenuates neurological symptoms in the experimental autoimmune encephalomyelitis model of multi
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ORIGINAL RESEARCH
Autoimmunity Highlights Open Access
Human placental extract attenuates neurological symptoms in the experimental autoimmune encephalomyelitis model of multiple sclerosis‑a putative approach in MS disease? Mir Hadi Jazayeri1,2*, Khadijeh barzaman1,2, Reza Nedaeinia3 , Tayebe Aghaie1 and Morteza Motallebnezhad1,2
Abstract Background: Different studies have demonstrated the anti-inflammatory effects of human placental extract both in vivo and in vitro. Considering the chronic inflammatory nature of multiple sclerosis (MS) disease, we examined whether or not the administration of human placental extract is able to attenuate the neurological symptoms detected in experimental autoimmune encephalomyelitis (EAE) model of MS. Methods: The injected myelin oligodendrocyte glycoprotein (MOG) induced EAE in mice, and treatment began from day 4 post-injection by intraperitoneal administration of 0.2 mg/kg human placental extract, repeated every other day up to day 31 post-injection. At the end of the treatment, luxol fast blue (LBS) staining and hematoxylin and eosin (H&E) staining were performed to evaluate the demyelination of neurons and inflammatory responses, respectively. Further assessed were the serum concentrations of IL-23 and IL-27. Results: The administration of human placental extract was able to significantly reduce the mean clinical score in EAE mice, decrease the pro-inflammatory process and attenuate neural demyelination. Moreover, while the serum concentration of IL-23 was significantly diminished in the EAE mice receiving human placental extract compared to the non-treated EAE group, IL-27 concentration was significantly increased. Conclusions: Our findings demonstrated the administration of human placental extract could significantly attenuate the neurological symptoms in the EAE model of MS in part through modulating the serum levels of IL-23 and IL-27 and enhancing neuroprotection and myelin repair. Keywords: Human placental extract, IL-23, IL-27, Multiple sclerosis, EAE
*Correspondence: [email protected] 1 Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Shahid Hemmat Highway, P.O Box: 14665‑354, Tehran 1449614535, Iran Full list of author information is available at the end of the article
Background Characterized by the progressive demyelination of neurons, multiple sclerosis is the most prevalent chronic inflammatory disorder of the nervous system, affecting more than 2 million individuals worldwide [1]. Among different pro-inflammatory mediators identified in the central nervous system (CNS), those related to interleukin-12 (IL-12) cytokine family containing IL-12, IL-23, IL-35, and IL-27 have shown to be in close association
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