Tolerogenic dendritic cells in experimental autoimmune encephalomyelitis, specific tolerance induction?
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POSTER PRESENTATION
Open Access
Tolerogenic dendritic cells in experimental autoimmune encephalomyelitis, specific tolerance induction? Carla Sellés-Moreno1,2,4*, Cristina Ramo-Tello2, Laia Grau-López2, Ricardo Pujol-Borrel3,4, Eva M Martínez-Cáceres1,4 From 7th European Workshop on Immune-Mediated Inflammatory Diseases Noordwijk aan Zee, the Netherlands. 28-30 November 2012 Background Specific cell therapy with tolerogenic Dendritic Cells (tolDCs) loaded with autoantigens is a promising tool for the attenuation of pathogenic T cells in autoimmune diseases such as Multiple Sclerosis (MS). The aim of this study was to analyse the in vitro effect of tolerogenic bone marrow derived DCs (BM-DCs) loaded with Myelin Oligodendrocyte Glycoprotein (MOG) 40-55 peptide from C57BL/6 mice, on the specific proliferation of splenocytes of mice with EAE. Methods Chronic EAE was induced in C57BL/6 mice by s.c. immunization with MOG40-55 emulsified in complete Freund’s adjuvant. Pertussis Toxin was injected i.v. at days 0 and 2 post-immunization to increase blood-brain-barrier permeability. Clinical score was daily measured based on tail/leg paralysis. TolBM-DCs were in vitro differenciated with GM-CSF in the presence of vitamin D3 (VD3) for 8 days. On day 7, maturation was induced with LPS. Viability, efficiency of differentiation and phenotype of tolBM-DCs were evaluated. To assess antigen specificity, tolBM-DCs were pulsed with MOG40-55, after the maturation stimulus (day 7) for 18h and cocultured with syngeneic splenocytes from mice with established EAE.
VD3. MOG40-55 loaded TolDCs showed to be poor stimulators of specific T cells of mice with EAE, compared to mBM-DCs.
Conclusion These results suggest that MOG-loaded TolDCs may be a powerful tool to induce specific T-cell hyporesponsiveness in mice with chronic MOG-induced EAE. Hence, treatment with tolDCs loaded with myelin peptides might be a potential therapy for EAE/MS. Author details 1 Immunobiology Laboratory For Research And Diagnostic Applications (LIRAD-BST), Badalona, Spain. 2Multiple Sclerosis Unit, Dept. of Neurosciences, Germans Trias i Pujol University Hospital (HUGTiP) and Health Science Research Institute (IGTP), Badalona, Spain. 3Dept. of Immunology, Hospital Vall d’Hebron, Barcelona, Spain. 4Universitat Autònoma Barcelona, (UAB), Spain. Published: 28 November 2012
doi:10.1186/1479-5876-10-S3-P27 Cite this article as: Sellés-Moreno et al.: Tolerogenic dendritic cells in experimental autoimmune encephalomyelitis, specific tolerance induction? Journal of Translational Medicine 2012 10(Suppl 3): P27.
Results TolBM-DCs displayed a semi-mature phenotype, exhibited by low levels of MHC class II and coestimulatory molecules (CD40, CD86) compared to control mature BM-DCs (mBM-DCs) differenciated in the absence of 1 Immunobiology Laboratory For Research And Diagnostic Applications (LIRAD-BST), Badalona, Spain Full list of author information is available at the end of the article
© 2012 Sellés-Moreno et al; licensee BioMed Central Ltd. This is an Open Access article
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