Hybridization of the redfish species Sebastes norvegicus and Sebastes mentella occurs in the Irminger Sea but not in the
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Hybridization of the redfish species Sebastes norvegicus and Sebastes mentella occurs in the Irminger Sea but not in the White Sea Aleksei Y. Rolskii1,2 · Valentina S. Artamonova3 · Alexander A. Makhrov3 Received: 25 June 2020 / Revised: 25 June 2020 / Accepted: 11 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Recently, Shum and Pampoulie (submitted) commented on our paper (Rolskii et al. 2020), which provided the first genetic confirmation of golden redfish Sebastes norvegicus in the White Sea. They argued that the White Sea redfish rather belong to a S. norvegicus × S. mentella hybrid. The objective of this reply is to respond to their criticisms. Keywords The White Sea · Sebastes norvegicus · Molecular methods Hyde and Vetter (2007) and Artamonova et al. (2013), based on the assumption that selected highly polymorphic mtDNA control region (D-loop) is species-specific marker, reached a consensus about the affinity of the majority of haplotypes to S. mentella and identified a compact cluster of haplotypes exclusively belonging to S. norvegicus. This cluster included, among others, the MR1 haplotype, the most frequent one in the S. norvegicus group (Artamonova et al. 2013). Later, Shum et al. (2017) concluded that the specific group of haplotypes, including MR1, belonged to S. mentella because the S. norvegicus samples examined by them had another haplotype that had not been identified before. A nuclear DNA marker (the second intron of the RP2 S7 gene) to clearly distinguish between S. mentella and S. norvegicus and their hybrids has been recently discovered This reply refers to the comment available online at https://doi. org/10.1007/s00300-020-02718-y. * Aleksei Y. Rolskii [email protected] 1
“Polar Branch of the “Russian Federal Research Institute of Fisheries and Oceanography” (“PINRO” Named After N.M. Knipovich), 6 Academician Knipovich Street, Murmansk, Russia 183038
2
Federal State Educational Institution of Higher Education “Murmansk State Technical University”, 13 Sportivnaya Street, Murmansk, Russia 183010
3
A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, 33 Leninsky prosp., Moscow, Russia 119071
(Rehbein 2013). The analysis of the RP2 S7 sequences in S. norvegicus samples with MR1 haplotype from different areas (Northwest Atlantic, n = 7; Norwegian Sea, n = 7) revealed that the RP2 S7 sequences of S. norvegicus were identical in all the cases and differed from the sequences of S. mentella. The latter ones had not only nucleotide substitutions but also four insertions (2 to 5 bp) and three deletions (1 to 37 bp). Thus, our conclusion that the redfish specimens with the MR1 haplotype belong to S. norvegicus is supported by the results from nuclear marker analysis (Rolskii 2016). Unfortunately, Shum et al. (2017) did not perform an analysis of redfish species-specific nuclear markers. In their Comment to Rolskii et al. (2020), Shum and Pampoulie (submitted) mentioned only microsatellite analysis. Whil
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