Identification of a delayed leaf greening gene from a mutation of pummelo
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entification of a delayed leaf greening gene from a mutation of pummelo 1,2†
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Hui-Wen Yu , Zhi-Hao Lu , Xia Wang , Dan Liu , Jia-Xian He , Xiao-Lin Jiang , 1,2 1 1 1* Ling-Jun Ke , Wen-Wu Guo , Xiu-Xin Deng & Qiang Xu 1
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Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics of Fujian Colleges and Universities, Minnan Normal University, Zhangzhou 363000, China Received June 24, 2020; accepted August 1, 2020; published online September 29, 2020
Delayed greening of young leaves is an unusual phenomenon of plants in nature. Citrus are mostly evergreen tree species. Here, a natural mutant of “Guanxi” pummelo (Citrus maxima), which shows yellow leaves at the young stage, was characterized to identify the genes underlying the trait of delayed leaf greening in plants. A segregating population with this mutant as the seed parent and a normal genotype as the pollen parent was generated. Two DNA pools respectively from the leaves of segregating seedlings with extreme phenotypes of normal leaf greening and delayed leaf greening were collected for sequencing. Bulked segregant analysis (BSA) and InDel marker analysis demonstrated that the delayed leaf greening trait is governed by a 0.3 Mb candidate region on chromosome 6. Gene expression analysis further identified a key candidate gene (Citrus Delayed Greening gene 1, CDG1) in the 0.3 Mb region, which showed significantly differential expression between the genotypes with delayed and normal leaf greening phenotypes. There was a 67 bp InDel region difference in the CDG1 promoter and the InDel region contains a TATA-box element. Confocal laser-scanning microscopy revealed that the CDG1-GFP fusion protein signals were co-localized with the chloroplast signals in the protoplasts. Overexpression of CDG1 in tobacco and Arabidopsis led to the phenotype of delayed leaf greening. These results suggest that the CDG1 gene is involved in controlling the delayed leaf greening phenotype with important functions in chloroplast development. citrus, pummelo, delayed leaf greening, chlorophyll Citation:
Yu, H.W., Lu, Z.H., Wang, X., Liu, D., He, J.X., Jiang, X.L., Ke, L.J., Guo, W.W., Deng, X.X., and Xu, Q. (2020). Identification of a delayed leaf greening gene from a mutation of pummelo. Sci China Life Sci 63, https://doi.org/10.1007/s11427-020-1790-0
INTRODUCTION Delayed leaf greening, which refers to the phenomenon that the young leaves are yellowish, reddish etc. for long time, and turn to green along with the maturation of leaves, is an uncommon phenomenon that affects the photosynthetic tissues and photosynthesis ability of plants in nature. Instead of being evergreen, the leaves show various colors along with seasonal changes. This phenomenon is different from the †Contributed equally to this work *Corresponding author (email: [email protected])
case of albino plants, which are commonly found in nature and can hardly
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