Identification of a genomic region controlling thermotolerance at flowering in maize using a combination of whole genomi
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ORIGINAL ARTICLE
Identification of a genomic region controlling thermotolerance at flowering in maize using a combination of whole genomic re‑sequencing and bulked segregant analysis Wei Zeng1 · Jian Shi1 · Chunhong Qiu1 · Yunhe Wang1 · Shamsur Rehman1 · Shuaishuai Yu1 · Shijie Huang1 · Chen He1 · Wanyi Wang1 · Hongyi Chen1 · Chen Chen1 · Chuanhong Wang1 · Zhen Tao1 · Peijin Li1 Received: 11 September 2019 / Accepted: 2 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message A novel genomic region controlling thermotolerance at flowering was identified by the combination of whole genomic re-sequencing and bulked segregant analysis in maize. Abstract The increasing frequency of extreme high temperature has brought a great threat to the development of maize throughout its life cycle, especially during the flowering phase. However, the genetic basis of thermotolerance at flowering in maize remains poorly understood. Here, we characterized a thermotolerant maize ecotype Abe2 and dissected its genetic basis using a F2:8 recombinant inbred line (RIL) population generated from a cross between Abe2 and B73. After continuous high temperature stress above 35 °C for 17 days, Abe2 and B73 show distinct leaf scorching phenotype under field conditions. To identify the genomic regions associated with the phenotypic variation, we applied a combination of whole genomic re-sequencing and bulked segregant analysis, and revealed 10,316,744 SNPs and 1,488,302 InDels between the two parental lines, and 2,693,054 SNPs and 313,757 InDels between the two DNA pools generated from the thermostolerant and the sensitive individuals of the RIL, of which, 108,655 and 17,853 SNPs may cause nonsynonymous variations. Finally, a 7.41 Mb genomic region on chromosome 1 was identified, and 7 candidate genes were annotated to participate in high temperature-related stress response. A candidate gene Zm00001d033339 encoding a serine/threonine protein kinase was proposed to be the most likely causative gene contributing to the thermotolerance at flowering by involving in stomatal movement (GO: 0010119) via Abscisic acid (ABA) pathway (KO04075). This work could provide an opportunity for gene cloning and pyramiding breeding to improve thermotolerance at flowering in maize.
Introduction Temperature is an important ecological factor for agriculture. Suitable environmental temperature is one of the necessary prerequisites to maximize crop yield. Extreme Communicated by Antonio Augusto Franco Garcia. Wei Zeng, Jian Shi, Chunhong Qiu contribute equally to this study. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03632-x) contains supplementary material, which is available to authorized users. * Peijin Li [email protected] 1
The National Engineering Laboratory of Crop Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
temperature can seriously affect crop growth and development. Over the past few decades,
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