Identification of a male sterile gene Ms in Brassica rapa L.
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Identification of a male sterile gene Ms in Brassica rapa L. Fengyan Shi & Zhiyong Liu & Nan Wang & Ying Zhao & Shiyao Dong & Jiaqi Zou & Hui Feng
Received: 10 October 2019 / Accepted: 4 June 2020 / Published online: 16 June 2020 # Springer Nature B.V. 2020
Abstract Male sterile lines are important for hybrid seed production in Brassica rapa. In our previous study, we obtained a multiple-allele inherited male sterile line of Chinese cabbage. To identify the male sterile gene Ms in B. rapa, we used B. rapa L. var. chinensis (‘AB03’; MsMs) and B. rapa var. purpuraria (‘AB04’; MsfMsf) as parents to construct an F2 segregating population, and mapped the Ms gene on chromosome A07 between Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01141-9) contains supplementary material, which is available to authorized users. F. Shi : Z. Liu : N. Wang : Y. Zhao : S. Dong : J. Zou : H. Feng (*) Department of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang 110866, China e-mail: [email protected] F. Shi e-mail: [email protected] Z. Liu e-mail: [email protected]
single sequence repeat (SSR) markers SSRY17 and SSRY22, which flank the gene at genetic distances of 0.11 cM and 0.31 cM, respectively. The interval between the two markers was 231.3 kb and comprised 21 genes. Whole-genome resequencing and sequence comparison revealed that only one gene, Bra015018, harbored an insertion–deletion (InDel) in an exon in the mapped interval. This InDel led to premature termination of translation due to generation of the stop codon TGA. Bra015018 was homologous to AT1G34355, which encoded a forkhead-associated (FHA) domaincontaining protein that was related to plant growth and floral development. Genotyping revealed that male sterility was fully co-segregated with the InDel. qRT-PCR analysis showed that Bra015018 was specifically expressed in the stamen. Consequently, we predicted that Bra015018 was a candidate gene of Ms. Keywords Brassica rapa . Male sterility . Fine mapping . Whole-genome resequencing . FHA domaincontaining protein
N. Wang e-mail: [email protected] Y. Zhao e-mail: [email protected] S. Dong e-mail: [email protected] J. Zou e-mail: [email protected]
Introduction Chinese cabbage is a typical allogamous Brassica crop and has significant heterosis. Because of its small flowers, it is difficult to artificially emasculate and propagate hybrid seeds. The usage of the male sterile line is an economic and reliable way for hybrid seed production.
Mol Breeding (2020) 40: 61
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Chinese cabbage is afflicted by two forms of male sterility: cytoplasmic male sterility (CMS) and genetic male sterility (GMS) (Vandermeer 1987). Because of complete stamen abortion and nearly no poor associated traits, GMS has attracted considerable attention among Chinese cabbage breeders. Most of the Chinese cabbage materials that have the GMS trait are controlled by recessive or dominant genes and can only be used as male sterile AB lines,
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