Identification of Anxiolytic Potential of Niranthin: In-vivo and Computational Investigations
- PDF / 2,035,066 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 162 Views
ORIGINAL ARTICLE
Identification of Anxiolytic Potential of Niranthin: In‑vivo and Computational Investigations Atul R. Chopade1 · Prakash M. Somade2 · Pratik P. Somade2 · Suraj N. Mali3 Received: 10 October 2020 / Accepted: 2 November 2020 © The Author(s) 2020
Abstract Anxiety is an unpleasant state, which can critically decrease the quality of life is often accompanied by nervous behaviour and rumination. Niranthin is a lignan isolated from various Phyllanthus sources. The literature survey on niranthin highlights wide ranges of the therapeutic potentials. In a present study, based on our previous investigations, we evaluated pure, isolated and characterized niranthin as an anxiolytic agent. The niranthin [6-[(2R,3R)-3-[(3,4-dimethoxyphenyl)methyl]-4-methoxy2-(methoxymethyl)butyl]-4-methoxy-1,3-benzodioxole] was purchased from commercial source and further subjected for assessment of its anxiolytic potentials using popular animal models including Elevated plus-maze model/test (EPM) and Light & Dark Exploration test (L&D). GABA-A receptor mediation was evaluated by pretreating the mice with the GABAA receptor antagonist Flumazenil before the EPM task. Molecular docking simulation studies (pdb id: 4COF) carried out by Vlife QSAR software showed that niranthin (docking score: − 62.1714 kcal/mol) have shown comparatively best docking score compared to the standard drug Diazepam (docking score: − 63.1568 kcal/mol). To conclude, Niranthin has probable potential in the management of anxiety disorder. Our in-silico and in-vivo analysis (indirectly) indicated the plausible role of GABA mediation for anxiolytic activity. Although, these studies are preliminary, future in depth experimental explorations will be required to use Niranthin as anti-anxiety drug in near future.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13659-020-00284-8) contains supplementary material, which is available to authorized users. * Atul R. Chopade [email protected] * Prakash M. Somade [email protected] 1
Department of Pharmacology, Rajarambapu College of Pharmacy, Kasegaon, Sangli, Maharashtra 415404, India
2
Dept. of Physiology, Krishna Institute of Medical Sciences, Karad, Maharashtra, India
3
Government College of Pharmacy, Karad, Maharashtra, India
13
Vol.:(0123456789)
A. R. Chopade et al.
Graphic Abstract
Keywords Pharmacology · Phytoconstituents · Niranthin · Lignan · Anxiolytic activity · EPM models
1 Introduction Anxiety, which has neurobiological, cognitive, and behavioral aspects, is one of the leading mental disorders of modern world experienced by children and adolescents [1–4]. It has been also noted that in certain conditions, stress and anxiety might find helpful as they will motivate individuals, but when it becomes excessive, it leads disturbances in psychological states of individuals. Anxiety is a central nervous system (CNS) disorder with negative emotional state, causing uneasiness, fear, etc. in response to factors perceived from in
Data Loading...
