Identification of Elite Population of Pandanus amaryllifolius Roxb. for Higher 2-Acetyl-1-pyrroline and Other Volatile C
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Identification of Elite Population of Pandanus amaryllifolius Roxb. for Higher 2-Acetyl-1-pyrroline and Other Volatile Contents by HS-SPME/GC-FID from Peninsular India Kantilal V. Wakte & Rahul L. Zanan & Ratnakar J. Thengane & Narendra Jawali & Altafhusain B. Nadaf
Received: 26 December 2011 / Accepted: 29 January 2012 / Published online: 19 February 2012 # Springer Science+Business Media, LLC 2012
Abstract The clonal populations of Pandanus amaryllifolius Roxb. from the western and eastern coastal regions of India were screened for 2-acetyl-1-pyrroline (2AP) and other volatile contents by HS-SPME/GC-FID. The analyses revealed that irrespective of locality, 19 volatile compounds were always detected in all population. 2AP, nonanal, phytol, 2hexenal, 2,6-nonadienal and hexanal were found to be the major contributors. The Palghar locality from Maharashtra state was found to harbour the highest amount of 2AP (12.25 ppm), nonanal (2.97 ppm) and 2-hexenal (25.70 ppm). Phytol, 2,6nonadienal and hexanal were highest in the Alibag locality (13.96 ppm), Chiplun locality (6.47 ppm) of Maharashtra state and the Bailhongal locality (6.25 ppm) of Karnataka state, respectively. Minor components accounted for 39.28% of the total volatiles. Besides 2AP, Palghar locality harboured ten other volatiles in higher amounts and hence was identified as an elite clonal population for the aroma contents.
Electronic supplementary material The online version of this article (doi:10.1007/s12161-012-9373-y) contains supplementary material, which is available to authorized users. K. V. Wakte : R. L. Zanan : R. J. Thengane : A. B. Nadaf (*) Department of Botany, University of Pune, Pune 411007, India e-mail: [email protected] N. Jawali Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
Keywords Pandanus amaryllifolius . 2-Acetyl-1-pyrroline . Elite population . Peninsular India
Introduction Pandanus amaryllifolius Roxb. has been identified as a rich natural source of principle basmati aroma compound 2-acetyl1-pyrroline (2AP). In our previous studies, in P. amaryllifolius, we have identified lower epidermal papillae as a site of storage of 2AP (Nadaf et al. 2006) and its detailed SEM studies have been carried out (Wakte et al. 2007). Further, developmental pattern of lower epidermal papillae has been traced out using in vitro regenerating plantlets (Wakte et al. 2009a). For extraction and quantification of 2AP and other volatiles, HS-SPME-GCFID based efficient method has been developed that revealed 21 new volatiles in this species (Wakte et al. 2010). A detailed survey by our group along peninsular India has revealed that the species is distributed more or less uniformly in South India with an exception of few states and commercially cultivated through lateral buds for raw leaves (Wakte et al. 2009b). The varied range of climatic conditions across the east and west coast of India provides a good source of collection of genotypes exposed to a range of environmental conditions. In the present study, 37 populatio
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