Identification of genes involved in phosphate solubilization and drought stress tolerance in chickpea symbiont Mesorhizo
- PDF / 983,425 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 29 Downloads / 189 Views
ORIGINAL PAPER
Identification of genes involved in phosphate solubilization and drought stress tolerance in chickpea symbiont Mesorhizobium ciceri Ca181 Akhilesh Yadav1 · Raghvendra Pratap Singh2 · Asha Lata Singh1 · Major Singh3,4 Received: 18 August 2020 / Revised: 6 October 2020 / Accepted: 23 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Chickpea plant root colonizing bacteria Mesorhizobium ciceri Ca181 promotes plant growth and development through symbiotic association with root nodules. The potentially beneficial effects on plants generated due to this bacterium are mineral nutrient solubilization, abiotic stress tolerance, and nitrogen-fixation, though the molecular mechanisms underlying these probiotic capacities are still largely unknown. Hence, this study aims to describe the molecular mechanism of M. ciceri Ca181 in drought stress tolerance and phosphorus solubilization. Here we have developed the transposon inserted mutant library of strain Ca181 and further screened it to identify the phosphorous solubilization and PEG-induced drought stress tolerance defective mutants, respectively. Resultantly, a total of four and three mutants for phosphorous solubilization and drought stress tolerance were screened and identified. Consequently, Southern blot confirmation was done for the cross verification of insertions and stability in the genome. Through the sequencing of each mutant, the interrupted gene was confirmed, and the finding revealed that the production of gluconic acid is necessary for phosphorus solubilization, while otsA, Auc, and Usp genes were involved in the mechanism of drought stress tolerance in M. ciceri Ca181. Keywords Chickpea · Drought · Mesorhizobium ciceri Ca181 · Mutant · Phosphate solubilization · Symbiont
Introduction Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02109-1) contains supplementary material, which is available to authorized users. * Akhilesh Yadav [email protected] * Major Singh [email protected] Asha Lata Singh [email protected] 1
Department of Botany, Banaras Hindu University, Varanasi 221005, India
2
Department of Biotechnology, Uttaranchal University, Dehradun 248007, India
3
Crop Improvement Division, ICAR-Indian Institute of Vegetable Research, Varanasi 221305, India
4
Present Address: ICAR-Directorate of Onion and Garlic Research, Pune 410505, India
Chickpea (Cicer arietinum L.), the most important leguminous plant species, belongs to the tribe Cicereae, subfamily Papilionaceae of the family Fabaceae. It is originated in southeastern Turkey, and the second-largest cultivated legumes around the world after soybean (Jukanti et al. 2012; Ladizinsky 1975). Chickpea (C. arietinum) provides food grains with more than 29% high-quality dietary protein, mostly cultivated in marginal soils with different types of stresses such as drought, heat, salinity, and mineral deficiency (Nandwani and Dudeja,
Data Loading...
