Identification of miR-7 as an oncogene in renal cell carcinoma
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ORIGINAL PAPER
Identification of miR-7 as an oncogene in renal cell carcinoma Zuhu Yu • Liangchao Ni • Duqun Chen • Qiang Zhang • Zhengming Su • Yadong Wang • Wenshui Yu • Xionghui Wu Jiongxian Ye • Shangqi Yang • Xianxin Li • Yongqing Lai
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Received: 23 April 2013 / Accepted: 28 May 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract MicroRNA-7 (miR-7) has been described as a tumor suppressor in several human cancers, but the results of a study to identify miRNAs associated with metastatic capability in breast cancer suggested that miR-7 may be characterized as an oncogene. The present study was to determine the expression and function of miR-7 in renal cell carcinoma. Quantitative real-time polymerase chain reaction was used to validate the expressions of miR-7 in 48 paired renal cell carcinomas (RCC) and normal tissues, based on the preliminary sequencing results of miRNAs. Furthermore, the impacts of miR-7 on cell migration, proliferation and apoptosis were analyzed using wound scratch assay, MTT and flow cytometry, respectively. The results demonstrated that miR-7 was up-regulated in RCC compared with normal tissues (p = 0.001). Down-regulation of miR-7 with synthesized inhibitor inhibited cell migration in vitro, suppressed cell proliferation and induced renal cancer cell apoptosis, prompting that miR-7
Zuhu Yu and Liangchao Ni contributed equally to this work. Yongqing Lai is the primary corresponding author. Z. Yu L. Ni D. Chen Q. Zhang Z. Su Y. Wang W. Yu X. Wu J. Ye S. Yang Y. Lai (&) X. Li (&) Department of Urology of Peking University Shenzhen Hospital, Institute of Urology of Shenzhen PKU-HKUST Medical Center, The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, 1120 Lianhua road, Shenzhen 518036, People’s Republic of China e-mail: [email protected] X. Li e-mail: [email protected] Z. Yu D. Chen Q. Zhang Z. Su Y. Wang W. Yu Anhui Medical University, Hefei 230032, Anhui, People’s Republic of China
could be characterized as an oncogene in RCC. The present study was the first to reveal that miR-7 was up-regulated in RCC and it played an important role in RCC by affecting cellular migration, proliferation and apoptosis. Further researches should be conducted to explore the roles and target genes of miR-7 in RCC and other cancers. Keywords Oncogene
MicroRNA miR-7 Renal cell carcinoma
Introduction Renal cell carcinomas (RCC) are tumors that originate in the renal cortex, accounting for approximately 3 % of adult malignancies and close to 90 % of all renal neoplasms (Jemal et al. 2010; Patel et al. 2012). The incidence of RCC is increasing in the United States while in China, the increase of RCC have surpassed the lung cancer with an average of 6 % annual increase in cases over the past 20 years (Patel et al. 2012; Zhao et al. 2010). About one third of patients have metastasis when initially diagnosed with RCC and up to 30 % of patients with clinically localized disease develop recurrence after surgery (Rouviere et al. 2006;
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