Identification of pollen and pistil polygalacturonases in Nicotiana tabacum and their function in interspecific stigma c
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ORIGINAL ARTICLE
Identification of pollen and pistil polygalacturonases in Nicotiana tabacum and their function in interspecific stigma compatibility Jugou Liao1 · Zhiyun Chen1 · Xuemei Wei1 · Keliang Tao1 · Jingwen Zhang1 · Xiaojun Qin1 · Zihui Pan1 · Wenguang Ma2 · Lei Pan3 · Shuai Yang3 · Mingqiao Wang4 · Xiaokun Ou1 · Suiyun Chen1 Received: 30 January 2020 / Accepted: 18 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The pollen and pistil polygalacturonases in Nicotiana tabacum were identified and found to regulate pollen tube growth and interspecific compatibility. Abstract Polygalacturonase (PG) is one of the enzymes catalyzing the hydrolysis of pectin. This process plays important roles in the pollen and pistil. In this research, the pollen and pistil PGs in Nicotiana tabacum (NtPGs) were identified, and their expression, localization and the potential function in the pollen and interspecific stigma incompatibility were explored. The results showed that 118 NtPGs were retrieved from the genome of N. tabacum. The phylogenetic tree and RT-qPCR analysis led to the identification of 10 pollen PGs; among them, two, seven and one showed specifically higher expression levels in the early development of anthers, during pollen maturation and in mature anthers, respectively, indicating their function difference. Immunofluorescence analysis showed that PGs were located in the cytoplasm of (1) mature pollen and (2) in vitro grown pollen tubes, as well as in the wall of in vivo grown pollen tubes. Four NtPGs in clade A were identified as the pistil PGs, and the pistil PGs were not found in clade E. Significantly higher PGs expression was recorded after incompatible pollination in comparison with the compatible stigma, indicating a potential function of PGs in regulating stigma incompatibility. The influence of PGs on pollen tube growth was explored in vitro and partly in vivo, showing that high PGs activity inhibited pollen tube growth. The application of PGs on the otherwise compatible stigma resulted in pollen tube growth inhibition or failure of germination. These results further supported that increased PGs expression in incompatible stigma might be partially responsible for the interspecific stigma incompatibility in Nicotiana. Keywords Nicotiana · Pollen · Pistil · Polygalacturonase · Interspecific stigma compatibility Communicated by Zhenbiao Yang. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00497-020-00393-x) contains supplementary material, which is available to authorized users. * Xiaokun Ou [email protected] * Suiyun Chen [email protected] 1
School of Ecology and Environmental Sciences, Biocontrol Engineering Research Center of Plant Diseases and Pests, Biocontrol Engineering Research Center of Crop Diseases & Pests, Yunnan University, Kunming 650091, Yunnan Province, China
2
Yunnan Academy of Tobacco Agricultural Sciences, Yuxi 653100, China
3
Yuxi China Tobacco Seed Co., Ltd., Yuxi 653100
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