Identification of up-regulated proteins potentially involved in the antagonism mechanism of Bacillus amyloliquefaciens G
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SHORT COMMUNICATION
Identification of up-regulated proteins potentially involved in the antagonism mechanism of Bacillus amyloliquefaciens G1 Haipeng Cao • Weidong Zheng • Shan He Hao Wang • Tu Wang • Liqun Lu
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Received: 25 December 2012 / Accepted: 5 March 2013 / Published online: 13 March 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract The use of Bacillus probiotics has been demonstrated as a promising method in the biocontrol of bacterial diseases in aquaculture. However, the molecular antibacterial mechanism of Bacillus still remains unclear. In order to explore the antibacterial mechanism of the potential antagonistic Bacillus amyloliquefaciens strain G1, comparative proteomics between B. amyloliquefaciens strain G1 and its non-antagonistic mutant strain was investigated. The 2-dimensional electrophoresis gel maps of their total extracted proteins were described and 42 different proteins were found to be highly expressed in strain G1 in comparison with those
in the mutant strain. 35 of these up-regulated proteins were successfully identified using MALDI-TOF-TOF MS and databank analysis, and their biological functions were analyzed through the KEGG database. The increased expression of these proteins suggested that high levels of energy metabolism, biosynthesis and stress resistance could play important roles in strain G1’s antagonism. To our knowledge, this is the first report on the proteins involved in the antagonism mechanism of B. amyloliquefaciens using a proteomic approach and the proteomic data also contribute to a better understanding of the molecular basis for the antagonism of B. amyloliquefaciens.
Haipeng Cao and Weidong Zheng are contributed equally to this work.
Keywords Bacillus amyloliquefaciens Antagonism mechanism Proteomics Up-regulated proteins
H. Cao (&) S. He H. Wang T. Wang L. Lu (&) Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education, Shanghai Ocean University, Shanghai 201306, People’s Republic of China e-mail: [email protected] L. Lu e-mail: [email protected] H. Cao S. He H. Wang T. Wang L. Lu National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, People’s Republic of China W. Zheng Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, People’s Republic of China
Introduction Bacterial antagonism is a common phenomenon for probiotic microbes in aquaculture and it plays a major role in reducing the incidence of opportunistic pathogens in aquatic animals’ gastrointestinal tract (Balca´zar et al. 2006). The mechanisms toward microbial pathogens include the competition for nutrients and space, the production of antibiotics and the production of cell wall-degrading enzymes (Berg et al. 2002). A few Bacillus species have been reported to be antagonistic against bacterial pathogens of fish and shellfish (Cao et al. 2011; Ran et al. 2012; Del’Duca et al. 2013)
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