A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacteri
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ORIGINAL ARTICLE
A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties Morahem Ashengroph 1
&
Seyedeh-Roya Hosseini 1
Received: 8 July 2020 / Revised: 14 October 2020 / Accepted: 16 October 2020 # Springer Nature Switzerland AG 2020
Abstract The aim of this study was to isolate and characterize marine bacterial strains capable of converting selenite to elemental selenium with the formation of Se nanoparticles (SeNPs). For the first time, a novel marine strain belonging to Bacillus amyloliquefaciens (GenBank accession no. MK392020) was isolated from the coast of the Caspian Sea and characterized based on its ability for transformation of selenite to SeNPs under aerobic conditions. The preliminary formation of SeNPs was confirmed via color changes and the products characterized by UV–Vis spectroscopy. The field-emission scanning electron microscopy (FESEM) together with energy-dispersive X-ray (EDX) analysis showed the presence of the spherical SeNPs on both the surface of the bacterial biomass and in the supernatant solution. Dynamic light scattering (DLS) analysis showed the SeNPs to have an average particle size (Z-average) around 45.4–68.3 nm. The X-ray diffraction (XRD) studies substantiated the amorphous nature of the biosynthesized SeNPs. Fourier-transform infrared spectroscopic (FTIR) studies of the SeNPs indicated typical proteinaceous and lipid-related bands as capping agents on the SeNPs. Different effective parameters corresponding the yield of SeNPs by B. amyloliquefaciens strain SRB04 were optimized under resting cell strategy. Results showed that the optimal process conditions for SeNP production were 2 mM of selenite oxyanion, 20 g/L of cell biomass, and 60 h reaction time. The synthesized SeNPs had a remarkable antibacterial activity on Staphylococcus aureus compared with chloramphenicol as a broad-spectrum antibiotic. Keywords Marine bacterium . SeNPs . B. amyloliquefaciens strain SRB04 . Resting cell . Antibacterial activity
Introduction Selenium is recognized as an essential element for plants and mammals. This non-metal element plays a significant role in improving the function of human body and preventing cellular damage by creating selenoproteins (Husen and Siddiqi 2014). Selenium exists in different oxidation states (− 2, 0, + 4, and + 6) and various forms, such as ionic selenite (SeO3−2) and selenate (SeO4−2), solid state (Se (0)), selenocysteine, and selenomethionine, in the environment (Wadhwani et al. 2016). The determinant factor in the toxicity of these various Supplementary Information The online version of this article (https:// doi.org/10.1007/s10123-020-00147-9) contains supplementary material, which is available to authorized users. * Morahem Ashengroph [email protected] 1
Department of Biological Science, Faculty of Science, University of Kurdistan, Kurdistan, Sanandaj, IR, Iran
forms is their degrees of solubility in water. Selenite with high solubility in water has the highest toxicity. H
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