IgG expression in trophoblasts derived from placenta and gestational trophoblastic disease and its role in regulating in
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IgG expression in trophoblasts derived from placenta and gestational trophoblastic disease and its role in regulating invasion Mei Yang • Chunfang Ha • Dan Liu • Yonghui Xu • Yuan Ma • Yufeng Liu Yan Nian
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Ó Springer Science+Business Media New York 2014
Abstract Immunoglobulin G (IgG) is an important humoral immune factor, which plays a role in innate immunity of the fetus. IgG immunoreactivity was often seen in trophoblasts of placenta. Traditionally, IgG in trophoblasts was believed to be transported from the maternal blood through neonatal Fc receptor (FcRn). Here, we explored the phenomenon of IgG expression and its role in regulating invasion in trophoblasts derived from normal placenta and gestational trophoblastic disease (GTD). IgG expression was detected with an emphasis on mRNA transcripts by using reverse transcription-polymerase chain reaction and hybridization in situ, besides evaluated at the protein level with immunohistochemistry and immunofluorescence. The migration and attachment of normal trophoblast cell line (TEV-1) and choriocarcinoma cell line (JAR) were inhibited with down-regulation of IgG expression. Methotrexate promoted the differentiation of
JAR cell line; however, it had little effect on the differentiation of TEV-1 cell line. IgG expression, migration, and attachment of JAR and TEV-1 cell lines were decreased in the presence of methotrexate. Furthermore, statistical analysis showed that the differences in migration and attachment were significant (P \ 0.05) for JAR cell line, while no significant difference was found for TEV-1 cell line. Collectively, these results confirmed that with the progression from normal placenta to GTD, the expression of IgG was increased in trophoblasts, which might actively promote the migration and attachment of trophoblasts as an important regulating factor. Keywords Immunoglobulin G Placenta Gestational trophoblastic disease Invasion Attachment
Introduction Mei Yang and Chunfang Ha have contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s12026-014-8486-3) contains supplementary material, which is available to authorized users. M. Yang Y. Ma Y. Liu Y. Nian Ningxia Medical University, Yinchuan, Ningxia, China C. Ha (&) D. Liu Department of Obstetrics and Gynecology in General Hospital, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, 804 Shengli South Street, Xingqing District, Yinchuan, Ningxia 750004, China e-mail: [email protected] Y. Xu Department of Pathology in General Hospital, Ningxia Medical University, Yinchuan, Ningxia, China
Human embryo implantation is a very complex process involving a series of delicately controlled interactions between fetal and maternal cells. Once embryo implantation begins, placental trophoblasts progress to own a moderate invasive phenotype, which makes trophoblasts invade decidua and even superficial uterine muscle under a specific temporal and spatial environment wit
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