Imaging of Stroke in Rodents Using a Clinical Scanner and Inductively Coupled Specially Designed Receiver Coils
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Annals of Biomedical Engineering ( 2020) https://doi.org/10.1007/s10439-020-02610-0
Original Article
Imaging of Stroke in Rodents Using a Clinical Scanner and Inductively Coupled Specially Designed Receiver Coils IGNACIO IN˜IGO-MARCO,1,2 JAVIER ISTU´RIZ,3 MIGUEL FERNA´NDEZ,4 MARIA J. NICOLAS,1,2 PABLO DOMI´NGUEZ,2,4 GORKA BASTARRIKA,2,4 MIGUEL VALENCIA,1,2 and MARI´A A. FERNA´NDEZ-SEARA 2,4 1 Neuroscience Program, CIMA, University of Navarra, Pamplona, Spain; 2IdiSNA, Navarra Institute for Health Research, Pamplona, Spain; 3Neos Biotec, Pamplona, Spain; and 4Radiology Department, Clı´ nica Universidad de Navarra, Pio XII, 36, 31008 Pamplona, Spain
(Received 6 April 2020; accepted 2 September 2020) Associate Editor Xiaoxiang Zheng oversaw the review of this article.
Abstract—Imaging of small laboratory animals in clinical MRI scanners is feasible but challenging. Compared with dedicated preclinical systems, clinical scanners have relatively low B0 field (1.5–3.0 T) and gradient strength (40–60 mT/m). This work explored the use of wireless inductively coupled coils (ICCs) combined with appropriate pulse sequence parameters to overcome these two drawbacks, with a special emphasis on the optimization of the coil passive detuning circuit for this application. A Bengal rose photothrombotic stroke model was used to induce cortical infarction in rats and mice. Animals were imaged in a 3T scanner using T2 and T1-weighted sequences. In all animals, the ICCs allowed acquisition of high-quality images of the infarcted brain at acute and chronic stages. Images obtained with the ICCs showed a substantial increase in SNR compared to clinical coils (by factors of 6 in the rat brain and 16–17 in the mouse brain), and the absence of wires made the animal preparation workflow straightforward.
FOV GRE ICC IP MR MRI PFA RARE RF ROI SNR TE T1 T2
Field of view Gradient echo Inductively coupled coil Intraperitoneally Magnetic resonance Magnetic resonance imaging Paraformaldehyde Rapid acquisition with relaxation enhancement Radio-frequency Region of interest Signal to noise ratio Echo time Longitudinal magnetization relaxation time Transverse magnetization relaxation time
Keywords—Preclinical imaging, Clinical scanner, Inductively coupled coil, Wireless coil, Stroke.
INTRODUCTION ABBREVIATIONS B0 B1 BOLD EPI
Main static magnetic field Rotating radio-frequency magnetic field Blood oxygen level dependent Echo planar imaging
Address correspondence to Marı´ a A. Ferna´ndez-Seara, Radiology Department, Clı´ nica Universidad de Navarra, Pio XII, 36, 31008 Pamplona, Spain. Electronic mail: [email protected] Miguel Valencia and Marı´ a A. Ferna´ndez-Seara coordinated equally this work.
Magnetic resonance imaging (MRI) is a powerful tool for disease diagnosis. It provides valuable structural information with high spatial resolution. To clearly discriminate between adjacent regions or between control and pathological tissue, the difference between desired and background signals must be high, that is, an adequate signal-to-noise ratio (SNR) mus
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