Immunoassay using dendritic Au-Pt nanoparticles as signal labels for detection of the biomarker of Burkholderia pseudoma
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Immunoassay using dendritic Au-Pt nanoparticles as signal labels for detection of the biomarker of Burkholderia pseudomallei Masoumeh Saber Zaeimian & David AuCoin & Xiaoshan Zhu
Received: 16 March 2020 / Accepted: 14 October 2020 # Springer Nature B.V. 2020
Abstract In this work, the peroxidase-like properties of dendritic Au-Pt nanoparticles (30~60 nm) were investigated and these nanoparticles were applied in the immunoassay as signal labels for the detection of capsular polysaccharides (CPS, ~ 300 kDa, expressed on the cell envelopes of Burkholderia pseudomallei causing melioidosis). The immunoassay adopts monoclonal anti-CPS antibodies. The assay limit of detection (LOD), which is calculated through the 3σ method, is at 0.36 ng/mL for CPS spiked in PBS with 5% milk, and at 0.88 ng/mL for CPS spiked in human serum. The results from this work show that the dendritic Au-Pt nanoparticles can produce similar assay performance in detecting CPS as HRP did. Considering these particles can be easily prepared and stable in environments (thus avoiding cold-chain based storage/transportation), they have potential to be applicable for both in-house
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11051-020-05054-8) contains supplementary material, which is available to authorized users. M. Saber Zaeimian : X. Zhu (*) Department of Electrical and Biomedical Engineering, University of Nevada Reno, Reno, NV, USA e-mail: [email protected] M. Saber Zaeimian : X. Zhu Biomedical Engineering Program, University of Nevada Reno, Reno, NV, USA D. AuCoin Department of Microbiology and Immunology, University of Nevada Reno, Reno, NV, USA
and in-field immunoassay development for the pathogen B. pseudomallei detection. Keywords Dendritic Au-Pt nanoparticles . Immunoassay . Burkholderia pseudomallei
Introduction Burkholderia pseudomallei is a gram-negative bacterial pathogen classified as tier 1 select agent by the US Centers for Disease Control and Prevention. People acquire melioidosis by inhaling dust or ingesting water contaminated with B. pseudomallei and when the contaminated soil/water comes in contact with an open wound of the skin. Melioidosis is apt to be mis-identified/diagnosed in its early stages due to its symptoms similar to other diseases like dengue and so on, and the mortality rate is high (> 40%). Although it was thought that B. pseudomallei can be commonly found in soil/ water in Southeast Asia and Northern Australia, it has been recently realized that melioidosis is an emerging infectious disease in many tropical developing countries (Limmathurotsakul et al. 2016). Currently, culture of B. pseudomallei from blood or other body fluids is the gold standard for diagnosis of melioidosis, but it takes 3 to 5 days before an evidencebased diagnosis can be made. Although the latex agglutination test is the only test readily available to clinicians in the field (Samosornsuk et al. 1999), it is used to agglutinate bacteria in blood specimens at a concentration of
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