Immunolocalisation of phosphorylated STAT3, interleukin 11 and leukaemia inhibitory factor in endometrium of women with
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BioMed Central
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Immunolocalisation of phosphorylated STAT3, interleukin 11 and leukaemia inhibitory factor in endometrium of women with unexplained infertility during the implantation window Evdokia Dimitriadis*1, Andrew M Sharkey2, Yee Lee Tan1, Lois A Salamonsen1 and J Robert A Sherwin3 Address: 1Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, VIC, 3168, Australia, 2Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP. UK and 3Department of Obstetrics and Gynaecology, University of Cambridge, The Rosie Hospital, Cambridge, CB2 2SW, UK Email: Evdokia Dimitriadis* - [email protected]; Andrew M Sharkey - [email protected]; Yee Lee Tan - [email protected]; Lois A Salamonsen - [email protected]; J Robert A Sherwin - [email protected] * Corresponding author
Published: 29 November 2007 Reproductive Biology and Endocrinology 2007, 5:44
doi:10.1186/1477-7827-5-44
Received: 14 September 2007 Accepted: 29 November 2007
This article is available from: http://www.rbej.com/content/5/1/44 © 2007 Dimitriadis et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Uterine receptivity and embryo implantation are critical in the establishment of pregnancy. The diagnosis of endometrial fertility requires more precise measurements of endometrial receptivity. Interleukin (IL-11) and leukemia inhibitory factor (LIF) are essential for murine implantation and signal via intracellular phosphorylation (p) of STAT3 in the endometrium. Both cytokines are present in the endometrium of women duiring the receptive window. Endometrial IL-11, IL-11 receptor alpha (IL-11Ralpha), LIF and pSTAT3 in women with primary unexplained infertility was compared to normal fertile women during the implantation window. Methods: LH timed endometrial biopsies (LH+6 to LH+10) were collected from women with unexplained infertility and normal fertility. pSTAT3, IL-11, IL-11Ralpha and LIF production was determined by immunohistochemistry. Staining intensity was determoned by two independent observers blind to the fertility status of the patient from whom the biopsy was taken. Staining intensity and heterogeneity in each of the endometrial compartments (epithelium; stroma, including decidualized stromal cells; and vasculature) was assessed. The Mann-Whitney U test was used to analyze IL-11, pSTAT3, IL-11Ralpha and LIF immunostaining intensities in the samples. Results: IL-11, IL-11Ralpha and LIF were present predominantly in glandular epithelium, whilst luminal epithelium showed patchy staining. pSTAT3 was present in both glandular epithelium and stroma. IL-11 and pSTAT3 immunostaining was significantly lower in glandular epithelium in infertile women compared to controls (P < 0.05) whilst IL-11Ralpha
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