Improvement of ethanol production in fed-batch fermentation using a mixture of sugarcane juice and molasse under very hi
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Improvement of ethanol production in fed‑batch fermentation using a mixture of sugarcane juice and molasse under very high‑gravity conditions Mariana Lopes Cruz1 · Miriam Maria de Resende1 · Eloízio Júlio Ribeiro1 Received: 6 June 2020 / Accepted: 11 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Ethanol fermentation in very high gravity (VHG) saves energy consumption for ethanol distillation. As the technology offers high ethanol yield and low waste generation and it can be operated at low cost, it could be more efficient at an industrial scale than other ethanol production methods. This work studied ethanol production using a fed-batch bioreactor with a working volume of 1.5 L. The main objective of this research was evaluate the effects of temperature, sugar concentration, and cellular concentration using a Central Composite Design (CCD). Experimental conditions were selected using the surface response technique obtained from the CCD, and the results were validated to test the reproducibility. The following operating conditions were selected: temperature of 27.0 °C, sugar concentration 300.0 g/L, and cell concentration 15.0% (v/v). Under these conditions, after 30 h of fermentation the ethanol concentration, productivity and yield were 135.0 g/L, 4.42 g/(L·h) and 90.0%, respectively. All sugar was completely consumed. Keywords Biofuel · Sugarcane · Temperature · Very high-gravity fermentation · Ethanol production optimization
Introduction The industrial process of ethanol production is constantly improving. One technology that is significantly changing the production of industrial ethanol is very high-gravity fermentation (VHG) or fermentations with high-sugar concentrations. Very high-gravity technology gives high ethanol concentrations of up to 120 g/L from moderately high sugar concentrations (≥ 250 g/L) [1]. The VHG fermentation process not only aims to increase the production of ethanol but also has technical, economic, and environmental advantages. High-density fermentation, a process intensification, is a technique that brings a number of potential benefits over conventional approaches, including higher ethanol production, lower cost of capital, and also minimization of bacterial contamination. VHG technology enables a reduction of process water requirements, thus * Miriam Maria de Resende [email protected] 1
Faculty of Chemical Engineering, Federal University of Uberlândia, Av. João Naves de Ávila, 2121, , Campus Santa Mônica ‑ Bloco 1K, Uberlândia, MG 38408‑144, Brazil
reducing the related distillation operational cost, vinasse generation, and its treatment cost, which results in significant energy savings [2]. As the technology offers high ethanol yield, low waste generation and low-cost operation, it could be more efficient at an industrial scale than other ethanol production methods [3]. The main advantage of this technology is the increase in ethanol concentration up to 15% (v/v), which consequently reduces the vinasse volume down to 5.4
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